抑肽酶体外抗凝特性的评估。

Evaluation of the anticoagulant properties of aprotinin in vitro.

作者信息

Mortier E, Ongenae M, Van Aken J, Den Blauwen N, Rolly G

机构信息

Department of Anaesthesiology, University Hospital Ghent, Belgium.

出版信息

Eur J Anaesthesiol. 1996 Sep;13(5):468-70. doi: 10.1046/j.1365-2346.1996.00991.x.

DOI:10.1046/j.1365-2346.1996.00991.x

PMID:8889419

Abstract

The interaction of aprotinin with normal coagulation was studied in blood samples obtained from 10 healthy subjects. Each sample was simultaneously tested in four different preparations: NaCl-treated blood: 0.03 mL 0.9% NaCl in 0.33 mL blood; aprotinin treated blood: 0.33 mL blood+aprotinin in 0.03 mL in aliquots to obtain a final blood concentration of respectively 50 KIU mL-1; 100 KIU mL-1 and 200 KIU mL-1. The coagulation process was analysed by thromboelastography. R-time, reflecting intrinsic coagulation, increased in a dose dependent manner between NaCl-treated and aprotinin-treated blood. These findings suggest a dose dependent impairment of intrinsic coagulation by aprotinin.

摘要

在从10名健康受试者采集的血样中研究了抑肽酶与正常凝血的相互作用。每个样本同时在四种不同制剂中进行测试：氯化钠处理的血液：0.33 mL血液中加入0.03 mL 0.9%氯化钠；抑肽酶处理的血液：0.33 mL血液 + 0.03 mL抑肽酶，分成等份以分别获得最终血液浓度为50 KIU/mL、100 KIU/mL和200 KIU/mL。通过血栓弹力图分析凝血过程。反映内源性凝血的R时间在氯化钠处理的血液和抑肽酶处理的血液之间呈剂量依赖性增加。这些发现表明抑肽酶对内源性凝血有剂量依赖性损害。