Chin E C, Senior M L, Shu H, Smith J S
Pioneer Hi-Bred International, USA.
Genome. 1996 Oct;39(5):866-73. doi: 10.1139/g96-109.
Microsatellites or simple sequence repeats, first demonstrated in human and other mammalian genomes, are being identified in many plant species. A database survey of 576 maize sequences from the GenBank and EMBL databases was made to determine the abundance of maize microsatellites. Two hundred potential microsatellites were identified. The relative abundance of the different repetitive motifs varied considerably and all possible dinucleotide and trinucleotide motif types were found. The three most abundant classes of microsatellites identified in this search were (AG/CT)n, (CCT/GGA)n, and (CCG/GGC)n repeats. Allelic variation was surveyed with 9 maize inbred lines representing diverse pedigrees. Amplification of DNA from these lines and analysis using high resolution agarose gels showed that 69 of the 200 potential microsatellites were polymorphic and yielded 2-4 alleles. A more complete screen of these loci against a wider array of maize germplasm using denaturing sequencing gels is now being conducted to more thoroughly evaluate these loci.
微卫星或简单序列重复序列最早在人类和其他哺乳动物基因组中得到证实，如今在许多植物物种中也被发现。对来自GenBank和EMBL数据库的576个玉米序列进行了数据库调查，以确定玉米微卫星的丰度。共鉴定出200个潜在的微卫星。不同重复基序的相对丰度差异很大，并且发现了所有可能的二核苷酸和三核苷酸基序类型。在此次搜索中鉴定出的最丰富的三类微卫星是(AG/CT)n、(CCT/GGA)n和(CCG/GGC)n重复序列。利用代表不同谱系的9个玉米自交系对等位基因变异进行了调查。从这些品系中扩增DNA并使用高分辨率琼脂糖凝胶进行分析，结果表明在200个潜在的微卫星中，有69个具有多态性，产生了2 - 4个等位基因。目前正在使用变性测序凝胶对更广泛的玉米种质进行这些位点的更全面筛选，以更彻底地评估这些位点。
