Becker J, Heun M
Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
Plant Mol Biol. 1995 Feb;27(4):835-45. doi: 10.1007/BF00020238.
Microsatellites have developed into a powerful tool for mapping mammalian genomes and first reports about their use in plants have been published. A database search of 228 barley sequences from GenBank and EMBL was made to determine which simple sequence repeat (SSR) motif prevails in barley. Nearly all types of SSRs were found. The (A)n and (T)n SSRs occurred more often than (C)n and (G)n for n > or = 10. Among the dinucleotide repeats, the (CG)n SSRs occurred least often. Trinucleotide repeats did not occur with n > 7 and there is no correlation between the GC content in the trinucleotide motifs and the number of observed SSRs. Analysing 15 different microsatellites with 11 barleys yielded 2.1 alleles per microsatellite. Sequencing 25 putative microsatellites showed that the resolution capacity of high-quality agarose gels was sufficient to determine differences of only three base pairs. Five microsatellites were mapped on three different chromosomes of a barley RFLP map.
微卫星已发展成为用于绘制哺乳动物基因组图谱的强大工具，且关于其在植物中应用的首批报告也已发表。对来自GenBank和EMBL的228个大麦序列进行数据库搜索，以确定哪种简单序列重复（SSR）基序在大麦中占主导地位。几乎发现了所有类型的SSR。当n≥10时，（A）n和（T）n SSR出现的频率高于（C）n和（G）n。在二核苷酸重复序列中，（CG）n SSR出现的频率最低。当n>7时，三核苷酸重复序列未出现，并且三核苷酸基序中的GC含量与观察到的SSR数量之间没有相关性。用11个大麦分析15个不同的微卫星，每个微卫星产生2.1个等位基因。对25个推定的微卫星进行测序表明，高质量琼脂糖凝胶的分辨能力足以确定仅三个碱基对的差异。五个微卫星被定位在大麦RFLP图谱的三条不同染色体上。
