2,4-二氯苯氧乙酸降解质粒pEST4011的2,4-二氯苯酚羟化酶基因tfdB和3,5-二氯儿茶酚1,2-双加氧酶基因tfdC的序列分析
Sequence analysis of the 2,4-dichlorophenol hydroxylase gene tfdB and 3,5-dichlorocatechol 1,2-dioxygenase gene tfdC of 2,4-dichlorophenoxyacetic acid degrading plasmid pEST4011.
作者信息
Kõiv V, Marits R, Heinaru A
机构信息
Institute of Molecular and Cell Biology, University of Tartu, Estonia.
出版信息
Gene. 1996 Oct 3;174(2):293-7. doi: 10.1016/0378-1119(96)00043-1.
Chlorocatechol 1,2-dioxygenase (CC12O) and 1,2-dichlorophenol hydroxylase (DCPH) encoding genes tfdC and tfdB are located on a 4.2-kb DNA fragment cloned from the 2,4-dichlorophenoxyacetic acid (2,4D) degrading plasmid pEST4011. The nucleotide sequences of tfdC and tfdB were determined. The DCPH is coded by a 1758-bp gene and CC12O is coded by a 762-bp gene. The deduced M(r) of these proteins are 64.09 kDa and 28.2 kDa, respectively. Expression analysis of tfdB and tfdC in Escherichia coli suggested that these genes form one operon, tfdCB.
编码氯儿茶酚1,2-双加氧酶（CC12O）和1,2-二氯苯酚羟化酶（DCPH）的基因tfdC和tfdB位于从降解2,4-二氯苯氧基乙酸（2,4-D）的质粒pEST4011克隆的一个4.2 kb DNA片段上。测定了tfdC和tfdB的核苷酸序列。DCPH由一个1758 bp的基因编码，CC12O由一个762 bp的基因编码。这些蛋白质的推导相对分子质量分别为64.09 kDa和28.2 kDa。tfdB和tfdC在大肠杆菌中的表达分析表明，这些基因形成一个操纵子tfdCB。
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