Rode H J, Little M, Fuchs P, Dörsam H, Schooltink H, de Inés C, Dübel S, Breitling F
German Cancer Research Center, Heidelberg, Germany.
Biotechniques. 1996 Oct;21(4):650, 652-3, 655-6, 658. doi: 10.2144/96214st02.
To provide an efficient means of coupling proteins, peptides and other suitable moieties to cells, we have constructed a retroviral expression vector for cell surface display of a single-chain antibody (scFv) against the hapten 4-ethoxymethylene-2-phenyl-oxazo-line-5-one (phOx). The hapten phOx can be easily conjugated to primary amino and sulfhydryl groups, thus providing points of attachment for the cell surface-bound anti-phOx scFv. This universal cell coupling system could prove to be particularly useful for anchoring monoclonal antibodies for tumor targeting and to present co-stimulatory molecules and other ligands (even mixtures) at the cell surface for gene therapy.
为了提供一种将蛋白质、肽和其他合适的部分与细胞偶联的有效方法，我们构建了一种逆转录病毒表达载体，用于在细胞表面展示针对半抗原4-乙氧基亚甲基-2-苯基-恶唑啉-5-酮（phOx）的单链抗体（scFv）。半抗原phOx可以很容易地与伯氨基和巯基偶联，从而为细胞表面结合的抗phOx scFv提供连接点。这种通用的细胞偶联系统可能被证明对于锚定用于肿瘤靶向的单克隆抗体以及在细胞表面呈现共刺激分子和其他配体（甚至混合物）以进行基因治疗特别有用。
