Goldstein N, Lewin T, Kamensky A, Dubinin V, Baumann S, Konstantinova O
Goldstein & Lewin Technology, GmbH, Dept. of Medical Research, Stahnsdorf, Germany.
Inflamm Res. 1996 Sep;45(9):473-8. doi: 10.1007/BF02252319.
The study examined the potentiation of the antinociceptive action of opioid analgesics produced by gaseous superoxide (GS) in the rat hind paw withdrawal test (PWT) and by GS or hydrogen peroxide (HP) in the formalin test. In the PWT, inhalation of GS for 50 minutes before i.p. injection of threshold doses of morphine (0.5 mg/kg) and trimeperidine (1.0 mg/kg) increased the threshold of nociception (TN) by a maximum of 43.0% (p < 0.05) and 113.4% (p < 0.01) respectively. The GS/trimeperidine-dependent increase in TN showed two peaks, the second of which could be suppressed by nialamide. Naloxone abolished the GS/ morphine-dependent increased in the TN. In the formalin test, a significant antinociceptive effect developed after GS inhalation or HP administration (intranasally, 2 x 5 microliters of 2 x 10(-5) mol/l solution in saline) in combination with low doses of Omnopon (0.06-0.75 mg/kg). These results suggest that both GS and HP potentiate the antinociceptive effects of opioid analgesics.
本研究在大鼠后爪缩足试验（PWT）中检测了气态超氧化物（GS）以及在福尔马林试验中检测了GS或过氧化氢（HP）对阿片类镇痛药抗伤害感受作用的增强效果。在PWT中，腹腔注射阈剂量的吗啡（0.5毫克/千克）和曲美哌啶（1.0毫克/千克）前50分钟吸入GS，分别使伤害感受阈值（TN）最高提高了43.0%（p < 0.05）和113.4%（p < 0.01）。GS/曲美哌啶依赖性的TN升高呈现两个峰值，其中第二个峰值可被尼亚酰胺抑制。纳洛酮消除了GS/吗啡依赖性的TN升高。在福尔马林试验中，吸入GS或给予HP（经鼻内，2×5微升2×10⁻⁵摩尔/升生理盐水溶液）与低剂量的阿片全碱（0.06 - 0.75毫克/千克）联合使用后产生了显著的抗伤害感受作用。这些结果表明，GS和HP均可增强阿片类镇痛药的抗伤害感受作用。
