Ishii K, Furuta T, Kasuya Y
Kyorin University, School of Health Sciences, Tokyo, Japan.
J Chromatogr B Biomed Appl. 1996 Aug 30;683(2):225-9. doi: 10.1016/0378-4347(96)00114-4.
An HPLC method for determining a flavonoid, naringin, and its metabolite, naringenin, in human plasma is presented for application to the pharmacokinetic study of naringin. Isocratic reversed-phase HPLC was employed for the quantitative analysis by using genistin (for naringin) or diadzein (for naringenin) as an internal standard and solid-phase extraction using s Sep-Pak t C18 cartridge. For the determination, HPLC was carried out using an Inertsil ODS-2 column (250 x 4.6 m I.D., 5 microns particle size). The mobile phases were acetonitrile-0.1 M ammonium acetate solution (20:80, v/v; pH 7.1) for naringin and acetonitrile-0.1 M ammonium acetate solution-acetic acid (30:69:1, v/v; pH 4.9) for naringenin. The flow-rate was 1 ml min-1. The analyses were performed by monitoring the wavelength of maximum UV absorbance at 280 nm for naringin and at 292 nm for naringenin. The detection limits on-column were about 0.2 ng for the two flavonoids.
本文介绍了一种用于测定人血浆中黄酮类化合物柚皮苷及其代谢产物柚皮素的高效液相色谱（HPLC）方法，该方法可应用于柚皮苷的药代动力学研究。采用等度反相HPLC进行定量分析，以染料木苷（用于柚皮苷）或黄豆苷元（用于柚皮素）为内标，并使用Sep-Pak t C18柱进行固相萃取。测定时，使用Inertsil ODS-2柱（250×4.6 mm内径，5微米粒径）进行HPLC分析。柚皮苷的流动相为乙腈-0.1 M醋酸铵溶液（20:80，v/v；pH 7.1），柚皮素的流动相为乙腈-0.1 M醋酸铵溶液-乙酸（30:69:1，v/v；pH 4.9）。流速为1 ml min-1。通过监测280 nm处柚皮苷和292 nm处柚皮素的最大紫外吸收波长进行分析。两种黄酮类化合物的柱上检测限约为0.2 ng。
