Nakashima K, Giannopoulou C, Andersen E, Roehrich N, Brochut P, Dubrez B, Cimasoni G
Division of Physiopathology and Periodontology, School of Dentistry, Medical Faculty, University of Geneva, Switzerland.
J Clin Periodontol. 1996 Sep;23(9):832-8. doi: 10.1111/j.1600-051x.1996.tb00620.x.
In order to examine the relationship of possible crevicular biochemical parameters to attachment loss (ALOSS), 330 sites from 8 untreated adult patients were monitored longitudinally at 3-month intervals, for up to 1 year. Attachment levels were measured with a force-sensing probe and an acrylic stent in duplicates at each study point. Crevicular samples were collected and used for the determination of the following 11 markers: number of polymorphonuclear leukocytes (PMNs), prostaglandin E2 (PGE2), osteocalcin (OC), alkaline phosphatase (ALP), collagenase (COL), beta-glucuronidase (BG), antigenic and functional elastase (AEL and FEL), alpha-1 antitrypsin (a1AT), alpha-2 macroglobulin (a2M) and aspartate aminotransferase (AST). 10 sites with ALOSS of > or = 1.5 mm per 3 months (active sites) and 43 sites with negligible changes (inactive sites) were identified. Total amounts of ALP, BG and COL were found to be significantly higher in active as compared to inactive sites, prior to significant ALOSS, without any significant differences in crevicular fluid volume and clinical indices. When biochemical parameters were expressed as ratios to the number of PMNs, PGE2/ PMNs was significantly elevated in active sites. The capacity of such individual parameters to distinguish between active and inactive sites was limited. However, linear discriminant analysis using total amounts of PGE2, COL, ALP, a2M, OC and AEL showed more significant diagnostic values (sensitivity: 80%, specificity: 91%). These findings suggest that the combination of several biochemical parameters in crevicular fluid could give more information to predict future clinical ALOSS.
为了研究龈沟液中可能的生化参数与附着丧失（ALOSS）之间的关系，对8例未经治疗的成年患者的330个位点进行了纵向监测，每隔3个月监测一次，最长监测1年。在每个研究点，使用力敏探针和丙烯酸支架对附着水平进行重复测量。收集龈沟液样本并用于测定以下11种标志物：多形核白细胞（PMN）数量、前列腺素E2（PGE2）、骨钙素（OC）、碱性磷酸酶（ALP）、胶原酶（COL）、β-葡萄糖醛酸酶（BG）、抗原性和功能性弹性蛋白酶（AEL和FEL）、α-1抗胰蛋白酶（a1AT）、α-2巨球蛋白（a2M）和天冬氨酸转氨酶（AST）。确定了每3个月附着丧失≥1.5 mm的10个位点（活动位点）和变化可忽略不计的43个位点（非活动位点）。在附着丧失显著之前，发现活动位点中ALP、BG和COL的总量显著高于非活动位点，而龈沟液体积和临床指标无显著差异。当生化参数表示为与PMN数量的比值时，活动位点中PGE2/PMN显著升高。这些单个参数区分活动位点和非活动位点能力有限。然而，使用PGE2、COL、ALP、a2M、OC和AEL总量进行线性判别分析显示出更显著的诊断价值（敏感性：80%，特异性：91%）。这些发现表明，龈沟液中几种生化参数的组合可以提供更多信息来预测未来的临床附着丧失。
