Bernstein J M, Boerst M, Hayes E R
Ann Otol Rhinol Laryngol. 1979 May-Jun;88(3 Pt 1):334-8. doi: 10.1177/000348947908800307.
Histochemical localization of sialomucins and sulfated polysaccharides have been demonstrated in the mucous membrane of the middle ear in different middle ear effusions. Inasmuch as a more neutral stain with alcian blue-PAS is still present after treatment with neuraminidase, it is strongly suggested that neutral polysaccharides coexist with neuraminic acid in the middle ear glycoproteins. Sulfated polysaccharides are present only in the mucoid effusions. The use of precise nomenclature for mucosubstances is strongly recommended and is outlined. The nature of the specificity of the histochemical stains is discussed.
在不同的中耳积液中,已证实涎黏蛋白和硫酸化多糖在中耳黏膜中的组织化学定位。由于用神经氨酸酶处理后仍存在更中性的阿尔辛蓝-过碘酸希夫染色,强烈提示中性多糖与神经氨酸在中耳糖蛋白中共存。硫酸化多糖仅存在于黏液样积液中。强烈建议并概述了对黏膜物质使用精确的命名法。讨论了组织化学染色特异性的性质。