Quan N, Zhang Z, Emery M, Lai E, Bonsall R, Kalyanaraman V S, Weiss J M
Department of Psychiatry and Behavioral Sciences, Emory University School of Medicine, Atlanta, Ga, USA.
Neuroimmunomodulation. 1996 Jan-Feb;3(1):56-61. doi: 10.1159/000097227.
We have previously reported that intracerebral infusion of recombinant human immunodeficiency virus envelope protein gp 120 induced interleukin-1 (IL-1) bioactivity in rat brain. In this study, we tested the ability of native gp 120 and gp 160 to induce IL-1 activity in rat brain and also examined whether altering the secondary and tertiary structures of these proteins by carboxymethylation could influence the IL-1-inducing effect of these peptides. Results showed that both native gp 120 and gp 160 can induce IL-1 activity in rat brain in vivo, and that intact secondary and tertiary structures of these proteins appear critical for this effect.
我们之前曾报道，向大鼠脑内注射重组人免疫缺陷病毒包膜蛋白gp 120可诱导大鼠脑内白细胞介素-1（IL-1）的生物活性。在本研究中，我们测试了天然gp 120和gp 160在大鼠脑内诱导IL-1活性的能力，并研究了通过羧甲基化改变这些蛋白质的二级和三级结构是否会影响这些肽诱导IL-1的效果。结果表明，天然gp 120和gp 160均可在体内诱导大鼠脑内IL-1活性，并且这些蛋白质完整的二级和三级结构对此效应似乎至关重要。
