阿泊拉霉素抗性作为分枝杆菌基因转移的选择标记
Apramycin resistance as a selective marker for gene transfer in mycobacteria.
作者信息
Paget E, Davies J
机构信息
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.
出版信息
J Bacteriol. 1996 Nov;178(21):6357-60. doi: 10.1128/jb.178.21.6357-6360.1996.
Abstract
We have explored the potential of using the apramycin resistance gene as a marker in mycobacterial gene transfer studies. Shuttle plasmids available for both electroporation and conjugation studies have been constructed, and we have successfully validated the use of the apramycin resistance gene as a component of cloning vectors for Mycobacterium smegmatis, M. bovis BCG, and M. tuberculosis.
摘要
我们已经探索了在分枝杆菌基因转移研究中使用安普霉素抗性基因作为标记的潜力。构建了可用于电穿孔和接合研究的穿梭质粒，并且我们已经成功验证了安普霉素抗性基因作为耻垢分枝杆菌、卡介苗和结核分枝杆菌克隆载体组成部分的用途。
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