来自黑暗链霉菌的氨基糖苷类抗性决定簇的克隆及其与其他放线菌相关基因的比较。
Cloning of aminoglycoside-resistance determinants from Streptomyces tenebrarius and comparison with related genes from other actinomycetes.
作者信息
Skeggs P A, Holmes D J, Cundliffe E
机构信息
Department of Biochemistry, University of Leicester, UK.
出版信息
J Gen Microbiol. 1987 Apr;133(4):915-23. doi: 10.1099/00221287-133-4-915.
PMID:3655737
Abstract
At least two aminoglycoside-resistance determinants from Streptomyces tenebrarius have been cloned separately in Streptomyces lividans. In each case, resistance (to kanamycin plus apramycin or to kanamycin plus gentamicin) was expressed at the level of the ribosome and involved specific methylation of 16S ribosomal RNA. Hybridization and restriction analysis revealed that related genes were present in other aminoglycoside-producing actinomycetes.
摘要
来自黑暗链霉菌的至少两个氨基糖苷抗性决定簇已分别在淡紫链霉菌中克隆。在每种情况下，（对卡那霉素加阿泊拉霉素或对卡那霉素加庆大霉素的）抗性在核糖体水平表达，并且涉及16S核糖体RNA的特异性甲基化。杂交和限制性分析表明，相关基因存在于其他产生氨基糖苷的放线菌中。
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