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IncP(α) 质粒 RP4 的 Tra2 核心对于大肠杆菌和淡紫链霉菌之间的属间交配是必需的。

The Tra2 core of the IncP(alpha) plasmid RP4 is required for intergeneric mating between Escherichia coli and Streptomyces lividans.

作者信息

Giebelhaus L A, Frost L, Lanka E, Gormley E P, Davies J E, Leskiw B

机构信息

Department of Biological Sciences, University of Alberta, Edmonton, Canada.

出版信息

J Bacteriol. 1996 Nov;178(21):6378-81. doi: 10.1128/jb.178.21.6378-6381.1996.

Abstract

Escherichia coli cells and Streptomyces mycelia are able to form close contacts in the absence of a conjugative system which might facilitate intergeneric plasmid transfer without the genes required for mating pair formation (Tra2) of the RP4 plasmid. The same Tra2 genes found to be essential for RP4 plasmid transfer, RSF1010 mobilization, and donor-specific phage propagation in E. coli were also required for intergeneric transfer between E. coli and Streptomyces lividans.

摘要

在没有接合系统的情况下,大肠杆菌细胞和链霉菌菌丝体能够形成紧密接触,这可能有助于种间质粒转移,而无需RP4质粒形成配对所需的基因(Tra2)。在大肠杆菌中发现对RP4质粒转移、RSF1010迁移和供体特异性噬菌体繁殖至关重要的相同Tra2基因,对于大肠杆菌和淡紫链霉菌之间的种间转移也是必需的。

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