大肠杆菌中TyrR介导的mtr和tyrP +3启动子激活的体外转录分析。
In vitro transcriptional analysis of TyrR-mediated activation of the mtr and tyrP+3 promoters of Escherichia coli.
作者信息
Yang J, Camakaris H, Pittard A J
机构信息
Department of Microbiology, The University of Melbourne, Parkville, Victoria, Australia.
出版信息
J Bacteriol. 1996 Nov;178(21):6389-93. doi: 10.1128/jb.178.21.6389-6393.1996.
Abstract
In order to understand the mechanism by which the TyrR protein activates transcription from the mtr and tyrP+3 promoters, we have carried out in vitro transcription experiments with supercoiled DNA templates. We have shown that addition of the histone-like protein HU or integration host factor (IHF) greatly inhibited the transcription from the mtr and tyrP+3 promoters. In the presence of phenylalanine, the wild-type TyrR protein, but not a mutant TyrR protein (activation negative), was able to relieve the HU- or IHF-mediated inhibition of transcription. In contrast, the alleviation of the HU- or IHF-mediated transcription inhibition by the wild-type TyrR protein did not occur when a mutant RNA polymerase with a C-terminally truncated alpha subunit was used to carry out the transcription reaction.
摘要
为了理解TyrR蛋白激活mtr和tyrP + 3启动子转录的机制，我们使用超螺旋DNA模板进行了体外转录实验。我们发现，添加类组蛋白HU或整合宿主因子（IHF）会极大地抑制mtr和tyrP + 3启动子的转录。在苯丙氨酸存在的情况下，野生型TyrR蛋白能够解除HU或IHF介导的转录抑制，而突变型TyrR蛋白（激活阴性）则不能。相反，当使用α亚基C末端截短的突变型RNA聚合酶进行转录反应时，野生型TyrR蛋白不会解除HU或IHF介导的转录抑制。
相似文献
1
In vitro transcriptional analysis of TyrR-mediated activation of the mtr and tyrP+3 promoters of Escherichia coli.大肠杆菌中TyrR介导的mtr和tyrP +3启动子激活的体外转录分析。
J Bacteriol. 1996 Nov;178(21):6389-93. doi: 10.1128/jb.178.21.6389-6393.1996.
2
Amino acid residues in the alpha-subunit C-terminal domain of Escherichia coli RNA polymerase involved in activation of transcription from the mtr promoter.大肠杆菌RNA聚合酶α亚基C末端结构域中参与mtr启动子转录激活的氨基酸残基。
J Bacteriol. 1997 Oct;179(19):6187-91. doi: 10.1128/jb.179.19.6187-6191.1997.
3
Mode of action of the TyrR protein: repression and activation of the tyrP promoter of Escherichia coli.TyrR蛋白的作用模式：大肠杆菌tyrP启动子的阻遏与激活
Mol Microbiol. 2004 Apr;52(1):243-56. doi: 10.1111/j.1365-2958.2003.03965.x.
4
The TyrR protein of Escherichia coli is a class I transcription activator.大肠杆菌的TyrR蛋白是一种I类转录激活因子。
J Bacteriol. 1995 Jan;177(1):238-41. doi: 10.1128/jb.177.1.238-241.1995.
5
Mechanism of repression of the aroP P2 promoter by the TyrR protein of Escherichia coli.大肠杆菌TyrR蛋白对aroP P2启动子的抑制机制。
J Bacteriol. 1999 Oct;181(20):6411-8. doi: 10.1128/JB.181.20.6411-6418.1999.
6
Analysis of an Escherichia coli mutant TyrR protein with impaired capacity for tyrosine-mediated repression, but still able to activate at sigma 70 promoters.对一种大肠杆菌突变型TyrR蛋白的分析，该蛋白酪氨酸介导的阻遏能力受损，但仍能在σ70启动子处激活。
Mol Microbiol. 1995 Aug;17(3):471-81. doi: 10.1111/j.1365-2958.1995.mmi_17030471.x.
7
Demonstration that the TyrR protein and RNA polymerase complex formed at the divergent P3 promoter inhibits binding of RNA polymerase to the major promoter, P1, of the aroP gene of Escherichia coli.证明在发散型P3启动子处形成的TyrR蛋白与RNA聚合酶复合物会抑制RNA聚合酶与大肠杆菌aroP基因的主要启动子P1的结合。
J Bacteriol. 1998 Oct;180(20):5466-72. doi: 10.1128/JB.180.20.5466-5472.1998.
8
Specific contacts between residues in the DNA-binding domain of the TyrR protein and bases in the operator of the tyrP gene of Escherichia coli.大肠杆菌TyrR蛋白DNA结合结构域中的残基与tyrP基因操纵子中的碱基之间的特异性接触。
J Bacteriol. 1999 Apr;181(8):2338-45. doi: 10.1128/JB.181.8.2338-2345.1999.
9
Critical base pairs and amino acid residues for protein-DNA interaction between the TyrR protein and tyrP operator of Escherichia coli.大肠杆菌TyrR蛋白与tyrP操纵基因之间蛋白质-DNA相互作用的关键碱基对和氨基酸残基
J Bacteriol. 1997 Feb;179(4):1051-8. doi: 10.1128/jb.179.4.1051-1058.1997.
10
Superimposition of tyrR protein-mediated regulation on osmoresponsive transcription of Escherichia coli proU in vivo.体内 tyrR 蛋白介导的调控对大肠杆菌 proU 渗透压响应转录的叠加作用。
J Bacteriol. 1998 Dec;180(24):6743-8. doi: 10.1128/JB.180.24.6743-6748.1998.
引用本文的文献
1
Activation by TyrR in Escherichia coli K-12 by Interaction between TyrR and the α-Subunit of RNA Polymerase.在大肠杆菌 K-12 中，TyrR 通过与 RNA 聚合酶α 亚基相互作用而被激活。
J Bacteriol. 2021 Sep 8;203(19):e0025221. doi: 10.1128/JB.00252-21.
2
Metabolic engineering for improving L-tryptophan production in Escherichia coli.代谢工程在大肠杆菌中提高 L-色氨酸生产的应用。
J Ind Microbiol Biotechnol. 2019 Jan;46(1):55-65. doi: 10.1007/s10295-018-2106-5. Epub 2018 Nov 13.
3
The TyrR transcription factor regulates the divergent akr-ipdC operons of Enterobacter cloacae UW5.TyrR转录因子调控阴沟肠杆菌UW5的双向akr-ipdC操纵子。
PLoS One. 2015 Mar 26;10(3):e0121241. doi: 10.1371/journal.pone.0121241. eCollection 2015.
4
Transcriptional regulation of multi-drug tolerance and antibiotic-induced responses by the histone-like protein Lsr2 in M. tuberculosis.结核分枝杆菌中类组蛋白Lsr2对多药耐受性和抗生素诱导反应的转录调控
PLoS Pathog. 2007 Jun;3(6):e87. doi: 10.1371/journal.ppat.0030087.
5
Mechanism of repression of the aroP P2 promoter by the TyrR protein of Escherichia coli.大肠杆菌TyrR蛋白对aroP P2启动子的抑制机制。
J Bacteriol. 1999 Oct;181(20):6411-8. doi: 10.1128/JB.181.20.6411-6418.1999.
6
Superimposition of tyrR protein-mediated regulation on osmoresponsive transcription of Escherichia coli proU in vivo.体内 tyrR 蛋白介导的调控对大肠杆菌 proU 渗透压响应转录的叠加作用。
J Bacteriol. 1998 Dec;180(24):6743-8. doi: 10.1128/JB.180.24.6743-6748.1998.
7
Demonstration that the TyrR protein and RNA polymerase complex formed at the divergent P3 promoter inhibits binding of RNA polymerase to the major promoter, P1, of the aroP gene of Escherichia coli.证明在发散型P3启动子处形成的TyrR蛋白与RNA聚合酶复合物会抑制RNA聚合酶与大肠杆菌aroP基因的主要启动子P1的结合。
J Bacteriol. 1998 Oct;180(20):5466-72. doi: 10.1128/JB.180.20.5466-5472.1998.
8
Amino acid residues in the alpha-subunit C-terminal domain of Escherichia coli RNA polymerase involved in activation of transcription from the mtr promoter.大肠杆菌RNA聚合酶α亚基C末端结构域中参与mtr启动子转录激活的氨基酸残基。
J Bacteriol. 1997 Oct;179(19):6187-91. doi: 10.1128/jb.179.19.6187-6191.1997.
9
Repression of the aroP gene of Escherichia coli involves activation of a divergent promoter.大肠杆菌aroP基因的阻遏涉及一个反向启动子的激活。
J Bacteriol. 1997 Jul;179(13):4213-8. doi: 10.1128/jb.179.13.4213-4218.1997.
本文引用的文献
1
Further genetic analysis of the activation function of the TyrR regulatory protein of Escherichia coli.大肠杆菌TyrR调节蛋白激活功能的进一步遗传分析。
J Bacteriol. 1996 Feb;178(4):1120-5. doi: 10.1128/jb.178.4.1120-1125.1996.
2
Evidence for two aromatic amino acid-binding sites, one ATP-dependent and the other ATP-independent, in the Escherichia coli regulatory protein TyrR.在大肠杆菌调节蛋白TyrR中存在两个芳香族氨基酸结合位点的证据，一个依赖ATP，另一个不依赖ATP。
Mol Microbiol. 1995 Aug;17(3):483-92. doi: 10.1111/j.1365-2958.1995.mmi_17030483.x.
3
Analysis of an Escherichia coli mutant TyrR protein with impaired capacity for tyrosine-mediated repression, but still able to activate at sigma 70 promoters.对一种大肠杆菌突变型TyrR蛋白的分析，该蛋白酪氨酸介导的阻遏能力受损，但仍能在σ70启动子处激活。
Mol Microbiol. 1995 Aug;17(3):471-81. doi: 10.1111/j.1365-2958.1995.mmi_17030471.x.
4
A mutational analysis of the structural basis for transcriptional activation and monomer-monomer interaction in the TyrR system of Escherichia coli K-12.大肠杆菌K-12 TyrR系统中转录激活和单体-单体相互作用结构基础的突变分析。
J Bacteriol. 1993 Mar;175(6):1777-84. doi: 10.1128/jb.175.6.1777-1784.1993.
5
A genetic analysis of various functions of the TyrR protein of Escherichia coli.大肠杆菌TyrR蛋白多种功能的遗传分析。
J Bacteriol. 1993 Mar;175(6):1767-76. doi: 10.1128/jb.175.6.1767-1776.1993.
6
7
Mutations in the tyrR gene of Escherichia coli which affect TyrR-mediated activation but not TyrR-mediated repression.大肠杆菌tyrR基因中的突变，这些突变影响TyrR介导的激活作用，但不影响TyrR介导的阻遏作用。
J Bacteriol. 1993 Oct;175(19):6372-5. doi: 10.1128/jb.175.19.6372-6375.1993.
8
Ligand-induced self-association of the Escherichia coli regulatory protein TyrR.配体诱导的大肠杆菌调节蛋白TyrR的自缔合。
J Mol Biol. 1994 May 6;238(3):309-18. doi: 10.1006/jmbi.1994.1294.
9
Architectural elements in nucleoprotein complexes: interchangeability of specific and non-specific DNA binding proteins.核蛋白复合物中的结构元件：特异性和非特异性DNA结合蛋白的互换性。
EMBO J. 1994 Oct 3;13(19):4536-48. doi: 10.1002/j.1460-2075.1994.tb06775.x.
10
The TyrR protein of Escherichia coli is a class I transcription activator.大肠杆菌的TyrR蛋白是一种I类转录激活因子。
J Bacteriol. 1995 Jan;177(1):238-41. doi: 10.1128/jb.177.1.238-241.1995.
Zotero 插件