马疱疹病毒1型的高度O-糖基化糖蛋白gp2由基因71编码。
The highly O-glycosylated glycoprotein gp2 of equine herpesvirus 1 is encoded by gene 71.
作者信息
Wellington J E, Allen G P, Gooley A A, Love D N, Packer N H, Yan J X, Whalley J M
机构信息
School of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia.
出版信息
J Virol. 1996 Nov;70(11):8195-8. doi: 10.1128/JVI.70.11.8195-8198.1996.
Abstract
There have been conflicting reports regarding the gene assignment of the high-molecular-mass envelope glycoprotein gp2 (gp300) of equine herpesvirus 1. Here, we provide an unequivocal demonstration that gp2 is encoded by gene 71. gp2 that was purified with a defining monoclonal antibody was cleaved internally to yield a 42-kDa protein encoded by gene 71. Amino acid composition data and N-terminal sequence analysis of a tryptic peptide identified gp2 as the product of equine herpesvirus 1 gene 71 with the SWISS-PROT database. Analysis of gp2's monosaccharide composition and the 42-kDa subunit showed that the high level of O glycosylation occurs on the serine/threonine-rich region upstream of the cleavage site.
摘要
关于马疱疹病毒1型高分子量包膜糖蛋白gp2（gp300）的基因定位，一直存在相互矛盾的报道。在此，我们提供了明确的证据表明gp2由基因71编码。用一种特异性单克隆抗体纯化的gp2在内部被切割，产生了由基因71编码的42 kDa蛋白。通过胰蛋白酶肽段的氨基酸组成数据和N端序列分析，并与SWISS-PROT数据库比对，确定gp2为马疱疹病毒1型基因71的产物。对gp2的单糖组成和42 kDa亚基的分析表明，高水平的O-糖基化发生在切割位点上游富含丝氨酸/苏氨酸的区域。
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本文引用的文献
1
Characterization of human plasma glycoproteins separated by two-dimensional gel electrophoresis.
Biotechnology (N Y). 1996 Jan;14(1):66-70. doi: 10.1038/nbt0196-66.
2
From proteins to proteomes: large scale protein identification by two-dimensional electrophoresis and amino acid analysis.从蛋白质到蛋白质组：通过二维电泳和氨基酸分析进行大规模蛋白质鉴定。
Biotechnology (N Y). 1996 Jan;14(1):61-5. doi: 10.1038/nbt0196-61.
3
Prediction of O-glycosylation of mammalian proteins: specificity patterns of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase.哺乳动物蛋白质O-糖基化的预测：UDP-N-乙酰半乳糖胺：多肽N-乙酰半乳糖胺基转移酶的特异性模式
Biochem J. 1995 Jun 15;308 ( Pt 3)(Pt 3):801-13. doi: 10.1042/bj3080801.
4
Evidence for involvement of equine herpesvirus 1 glycoprotein B in cell-cell fusion.马疱疹病毒1型糖蛋白B参与细胞间融合的证据。
Arch Virol. 1996;141(1):167-75. doi: 10.1007/BF01718598.
5
N-terminal sequence analysis of equine herpesvirus 1 glycoproteins D and B and evidence for internal cleavage of the gene 71 product.马疱疹病毒1型糖蛋白D和B的N端序列分析以及基因71产物内部切割的证据。
J Gen Virol. 1996 Jan;77 ( Pt 1):75-82. doi: 10.1099/0022-1317-77-1-75.
6
High molecular weight polypeptide bands specific for equine herpesvirus 4.针对马疱疹病毒4的高分子量多肽条带。
Vet Microbiol. 1995 Sep;46(1-3):203-11. doi: 10.1016/0378-1135(95)00084-n.
7
Analysis of the nucleotide sequence of five genes at the left end of the unique short region of the equine herpesvirus 4 genome.马疱疹病毒4型基因组独特短区域左端五个基因的核苷酸序列分析。
Arch Virol. 1993;128(1-2):143-54. doi: 10.1007/BF01309795.
8
The open reading frames 1, 2, 71, and 75 are nonessential for the replication of equine herpesvirus type 1 in vitro.
Virology. 1994 Mar;199(2):448-52. doi: 10.1006/viro.1994.1143.
9
Identification and characterization of the protein product of gene 71 in equine herpesvirus 1.马疱疹病毒1型中基因71的蛋白质产物的鉴定与特性分析
J Gen Virol. 1994 Nov;75 ( Pt 11):3117-26. doi: 10.1099/0022-1317-75-11-3117.
10
Identification of the envelope surface glycoproteins of equine herpesvirus type 1.1型马疱疹病毒包膜表面糖蛋白的鉴定
J Gen Virol. 1982 Dec;63(2):481-5. doi: 10.1099/0022-1317-63-2-481.
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