Zheng M, Love D N, Sabine M
Stanford Consulting Laboratory, Rydalmere, NSW, Australia.
Vet Microbiol. 1995 Sep;46(1-3):203-11. doi: 10.1016/0378-1135(95)00084-n.
Serum neutralisation (SN) and immunoblotting were used in attempts to distinguish between natural infections with the closely related viruses equine herpesvirus 1 (EHV-1) and equine herpes-virus 4 (EHV-4). Horse sera (n = 323) collected in 1990 from studs with no experience of EHV-1 abortions as well as 197 sera collected in 1992 from studs with a history of EHV-1 abortions were tested by SN. The two groups differed in the proportion with measurable EHV-1 antibody, the 1992 group being significantly higher. Both groups had high proportions with EHV-4 antibody and no serum had antibody to EHV-1 alone. Pools of positive sera were prepared as probes in immunoblots. High molecular weight (> 200 kDa) polypeptide bands specific for EHV-4 were detected. No bands specific for EHV-1 only were found. The specific EHV-4 polypeptides shared some properties with gp2 of EHV-1.
采用血清中和试验（SN）和免疫印迹法来区分密切相关的病毒——马疱疹病毒1型（EHV - 1）和马疱疹病毒4型（EHV - 4）的自然感染情况。对1990年从无EHV - 1流产病史的种马场采集的323份马血清以及1992年从有EHV - 1流产病史的种马场采集的197份马血清进行了SN检测。两组血清中可检测到EHV - 1抗体的比例有所不同，1992年的组比例显著更高。两组中EHV - 4抗体比例都很高，且没有血清仅含有EHV - 1抗体。将阳性血清混合作为免疫印迹的探针。检测到了EHV - 4特有的高分子量（> 200 kDa）多肽条带。未发现仅为EHV - 1特有的条带。EHV - 4的特异性多肽与EHV - 1的gp2有一些共同特性。
