Léon-Kloosterziel K M, Gil M A, Ruijs G J, Jacobsen S E, Olszewski N E, Schwartz S H, Zeevaart J A, Koornneef M
Department of Genetics, Wageningen Agricultural University, The Netherlands.
Plant J. 1996 Oct;10(4):655-61. doi: 10.1046/j.1365-313x.1996.10040655.x.
Novel Arabidopsis mutants with lowered levels of endogenous abscisic acid (ABA) were isolated. These were selected in a screen for germination in the presence of the gibberellin biosynthesis inhibitor paclobutrazol. Another mutant was isolated in a screen for NaCl tolerance. The ABA-deficiency was caused by two monogenic, recessive mutations, aba2 and aba3, that were both located on chromosome 1. The mutants showed a phenotype that is known to be characteristic for ABA-deficiency: a reduced seed dormancy and excessive water loss, leading to a wilty phenotype. Double mutant analysis, combining different aba mutations, indicated the leaky nature of the mutations.
分离出了内源脱落酸（ABA）水平降低的拟南芥新突变体。这些突变体是在赤霉素生物合成抑制剂多效唑存在的情况下筛选萌发时获得的。另一个突变体是在耐NaCl筛选中分离得到的。ABA缺乏是由两个单基因隐性突变aba2和aba3引起的，这两个突变都位于1号染色体上。这些突变体表现出已知的ABA缺乏特征性表型：种子休眠降低和水分过度流失，导致萎蔫表型。结合不同aba突变的双突变分析表明了这些突变的渗漏性质。
