马疱疹病毒1型糖蛋白D在哺乳动物细胞系中的表达与特性分析

Expression and characterization of equine herpesvirus 1 glycoprotein D in mammalian cell lines.

作者信息

Wellington J E, Lawrence G L, Love D N, Whalley J M

机构信息

School of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia.

出版信息

Arch Virol. 1996;141(9):1785-93. doi: 10.1007/BF01718301.

DOI:10.1007/BF01718301

PMID:8893800

Abstract

Equine herpesvirus 1 glycoprotein D (EHV-1 gD) expressed constitutively in mammalian cell lines had similar electrophoretic mobility to gD produced in EHV-1 infected cells but lacked a possibly complexed higher molecular weight form seen in the latter. Recombinant gD was N-terminally cleaved at the same site as gD in EHV-1 infected cells and expression was associated with enhanced levels of cell-cell fusion, indicating a role for EHV-1 gD in cell-to-cell transmission of virus.

摘要

在哺乳动物细胞系中组成性表达的马疱疹病毒1型糖蛋白D（EHV-1 gD）与EHV-1感染细胞中产生的gD具有相似的电泳迁移率，但缺乏在后者中可见的可能与之复合的高分子量形式。重组gD在N端与EHV-1感染细胞中的gD在相同位点被切割，并且其表达与细胞间融合水平的提高相关，表明EHV-1 gD在病毒的细胞间传播中发挥作用。