Whalley J M, Love D N, Tewari D, Field H J
School of Biological Sciences, Macquarie University, Sydney, NSW, Australia.
Vet Microbiol. 1995 Sep;46(1-3):193-201. doi: 10.1016/0378-1135(95)00083-m.
A series of recombinant baculoviruses containing genes for glycoproteins C, D, H and L of equine herpesvirus 1 (EHV-1) have been constructed, and the EHV-1 products characterised by gel electrophoresis and immunoblotting. The EHV-1 glycoproteins expressed in insect cells were similar but not identical in apparent sizes to those expressed in EHV-1 infected mammalian cells. Each of the EHV-1 products was recognised by convalescent equine sera, indicating that they were all targets for an equine immune response. Mice immunised with baculovirus-expressed EHV-1 gD and gC acquired an enhanced ability to clear challenge EHV-1 from respiratory tissues, in association with both neutralising antibody and cell mediated immune responses.
已经构建了一系列含有马疱疹病毒1型（EHV-1）糖蛋白C、D、H和L基因的重组杆状病毒，并通过凝胶电泳和免疫印迹对EHV-1产物进行了表征。在昆虫细胞中表达的EHV-1糖蛋白在表观大小上与在EHV-1感染的哺乳动物细胞中表达的糖蛋白相似但不完全相同。EHV-1的每种产物都能被康复马血清识别，这表明它们都是马免疫反应的靶点。用杆状病毒表达的EHV-1 gD和gC免疫的小鼠，与中和抗体和细胞介导的免疫反应相关，获得了增强的从呼吸道组织清除攻击型EHV-1的能力。
