在系统性红斑狼疮中寻找爱泼斯坦-巴尔病毒和C型肿瘤病毒。

Search for Epstein-Barr and type C oncornaviruses in systemic lupus erythematosus.

作者信息

Hicks J T, Aulakh G S, McGrath P P, Washington G C, Kim E, Alepa F P

出版信息

Arthritis Rheum. 1979 Aug;22(8):845-57. doi: 10.1002/art.1780220807.

DOI:10.1002/art.1780220807

Abstract

Lymphoblastoid cell lines were derived from patients with active systemic lupus erythematosus by allowing spontaneous transformation of peripheral B lymphocytes (B cells) harboring endogenous Epstein-Barr virus or by superinfecting peripheral lymphocytes with exogeneous Epstein-Barr virus. Results of extensive studies aimed at identifying type C oncornaviruses in these lymphoblastoid cells were entirely negative by electron microscopy, DNA-DNA hybridization, reverse transcriptase assays, and cocultivation experiments. These results do not support the postulated association of oncornavirus infection in human systemic erythematosus.

摘要

通过使携带内源性爱泼斯坦-巴尔病毒的外周B淋巴细胞（B细胞）自发转化，或用外源性爱泼斯坦-巴尔病毒超感染外周淋巴细胞，从活动性系统性红斑狼疮患者中获得淋巴母细胞样细胞系。通过电子显微镜、DNA-DNA杂交、逆转录酶测定和共培养实验，旨在鉴定这些淋巴母细胞样细胞中C型肿瘤病毒的广泛研究结果均为阴性。这些结果不支持人类系统性红斑狼疮中肿瘤病毒感染的假设关联。