McManus D P, Bowles J
Molecular Parasitology Unit, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research, Brisbane, Australia.
Int J Parasitol. 1996 Jul;26(7):687-704. doi: 10.1016/0020-7519(96)82612-9.
A wide range of approaches is available to parasitologists to aid in specific parasite identification and to formulate phylogenetic relationships. This review emphasises the usefulness of molecular genetic techniques, especially DNA-based procedures, in addressing problems of identification, characterisation and phylogeny of parasites. It should be stressed that an understanding of the various DNA approaches, techniques and target genes most likely to be effective in addressing key issues in diagnostic parasitology and systematics is still developing. Nevertheless, DNA methods clearly have great potential with regard to specificity and sensitivity, and applications will increase further with technological advance. Indeed, because of the minimal requirements for material, PCR-based methods especially should prove of immense value in future studies with parasites.
寄生虫学家有多种方法可用于辅助特定寄生虫的鉴定并构建系统发育关系。本综述强调了分子遗传学技术，尤其是基于DNA的方法，在解决寄生虫鉴定、特征描述和系统发育问题方面的实用性。应该强调的是，对于各种最有可能有效解决诊断寄生虫学和系统学关键问题的DNA方法、技术和靶基因的理解仍在不断发展。然而，DNA方法在特异性和敏感性方面显然具有巨大潜力，并且随着技术进步其应用将进一步增加。事实上，由于对材料的要求极低，基于PCR的方法在未来寄生虫研究中尤其应具有巨大价值。
