Histopathologic Alterations between and Genotypes of Human Cystic Echinococcosis Cysts in Shiraz, Iran.

BACKGROUND

We aimed to determine the genotypes of () using DNA regions within the () mitochondrial genes in formalin-fixed paraffin-embedded (FFPE) isolates of human cystic echinococcosis cysts (CE cysts) and compare their histopathologic alterations.

METHODS

Out of 135 samples, 21 high-quality PCR positive samples were selected for sequencing and were deposited into GenBank database. Moreover, histopathological changes of (G1 genotype) and (G6 genotype) cases were also compared.

RESULTS

Based on the sequencing results, 16 cases were diagnosed as (G1-G3 genotype) and 5 cases as (G6 genotype). Five haplotypes of were identified from 21 sequences. The histopathological alterations in both genotypes showed laminated layer of CE without inflammatory cells. In a few cases of the G6 genotype, neutrophils in the outer cuticular layer with mild vascular and congestion were observed. Cell debris with multiple areas of necrosis, as well as scanty lymphoplasma cells in the outer cuticular layer were observed in G1-G3 genotype cases. So, the histopathological differences between the two genotypes are not noticeable enough to be differentiated by microscopical observations.

CONCLUSION

(G1-G3) and (G6 genotype) are prevalent among CE patients. In general, five haplotypes were identified by genes analysis. The histopathological differences between the two genotypes have not been so big to be differentiated by microscopic observations.