流动注射荧光法测定药物制剂中的青霉胺和硫普罗宁。

Flow-injection fluorimetric determination of penicillamine and tiopronin in pharmaceutical preparations.

作者信息

Pérez Ruiz T, Martínez-Lozano C, Tomás V, Sidrach de Cardona C

机构信息

Department of Analytical Chemistry, University of Murcia, Spain.

出版信息

J Pharm Biomed Anal. 1996 Oct;15(1):33-8. doi: 10.1016/0731-7085(96)01821-3.

DOI:10.1016/0731-7085(96)01821-3

PMID:8895074

Abstract

Two flow-injection methods for the fluorimetric determination of penicillamine and tiopronin are proposed. The procedures are based on the oxidation of these drugs by thallium(III). In hydrochloric acid medium the fluorescence of thallium(I) formed in the oxidation of penicillamine or tiopronin is monitored using excitation and emission wavelengths of lambda ex = 227 nm and lambda em = 419 nm respectively. Linear calibration graphs were obtained between 3 x 10(-7) and 8 x 10(-6) M for penicillamine and between 8 x 10(-7) and 2 x 10(-5) M for tiopronin with sampling frequencies of 90 and 45 samples h-1 respectively. The relative standard deviations were in the ranges 0.48-0.29% for penicillamine and 1.04-0.31% for tiopronin. The applicability of the method to the determination of both drugs in pharmaceutical preparations was demonstrated by investigating the effect of potential interferences and by analysis of commercial preparations.

摘要

提出了两种用于荧光法测定青霉胺和硫普罗宁的流动注射方法。该方法基于这些药物被铊（III）氧化。在盐酸介质中，分别使用激发波长λex = 227 nm和发射波长λem = 419 nm监测青霉胺或硫普罗宁氧化过程中形成的铊（I）的荧光。青霉胺在3×10（-7）至8×10（-6）M之间以及硫普罗宁在8×10（-7）至2×10（-5）M之间获得线性校准曲线，采样频率分别为90和45个样品/小时。青霉胺的相对标准偏差在0.48 - 0.29%范围内，硫普罗宁的相对标准偏差在1.04 - 0.31%范围内。通过研究潜在干扰的影响和分析市售制剂，证明了该方法在药物制剂中测定这两种药物的适用性。