Lohmander L S, Yoshihara Y, Roos H, Kobayashi T, Yamada H, Shinmei M
Department of Orthopedics, Lund University Hospital, Sweden.
J Rheumatol. 1996 Oct;23(10):1765-9.
To determine in a cross sectional study the concentrations in joint fluid of the C-propeptide of collagen II (pCol II-C) in patients with knee injury and developing osteoarthritis (OA).
Synovial fluid (SF) samples were collected from knees of healthy volunteers, from patients with injury to the knee causing lesions of the anterior cruciate ligament and/or menisci, and from patients with posttraumatic or primary OA. Concentrations of pCol II-C were determined by enzyme immunoassay with a polyclonal antiserum against the bovine propeptide.
The median concentration of pCol II-C in joint fluid in the reference group was 1.3 ng/ml (range 0.1-5.7 ng/ml). Median concentrations of pCol II-C in joint fluid were increased 2-4-fold in all 3 study groups over that in the reference group. Very high concentrations of propeptide were noted in samples from patients younger than about 18 years. Propeptide concentrations were increased after knee injury, with a suggested peak at about 1-4 years evident for patients with cruciate ligament injury. pCol II-C levels were increased at all stages of OA development, except in the most advanced phases.
The increased levels of pCol II-C in SF may reflect an increased rate of synthesis of collagen II in the joint cartilage of patients with knee injury and developing OA. The increase reaches a maximum well before radiographic changes indicative of OA are apparent, and occurs during a disease phase characterized by signs of increased degradation of collagen II, aggrecan, and other matrix components. Further studies of markers of matrix metabolism of cartilage, bone, and other joint tissues in human and animal models of OA may aid in the identification of process markers, individuals at risk, and new therapeutic targets.
在一项横断面研究中，测定膝关节损伤且正在发展为骨关节炎（OA）患者关节液中Ⅱ型胶原C端前肽（pCol II - C）的浓度。
从健康志愿者、膝关节损伤导致前交叉韧带和/或半月板损伤的患者以及创伤后或原发性OA患者的膝关节中采集滑液（SF）样本。使用针对牛前肽的多克隆抗血清通过酶免疫测定法测定pCol II - C的浓度。
参照组关节液中pCol II - C的中位浓度为1.3 ng/ml（范围0.1 - 5.7 ng/ml）。所有3个研究组关节液中pCol II - C的中位浓度均比参照组升高了2 - 4倍。在年龄小于约18岁患者的样本中发现前肽浓度非常高。膝关节损伤后前肽浓度升高，对于交叉韧带损伤患者，在约1 - 4年时出现明显的峰值。除了最晚期阶段，在OA发展的各个阶段pCol II - C水平均升高。
SF中pCol II - C水平升高可能反映了膝关节损伤且正在发展为OA患者关节软骨中Ⅱ型胶原合成速率增加。在影像学上显示OA的变化明显之前，这种增加就已达到最大值，并且发生在以Ⅱ型胶原、聚集蛋白聚糖和其他基质成分降解增加为特征的疾病阶段。对OA的人类和动物模型中软骨、骨和其他关节组织的基质代谢标志物进行进一步研究，可能有助于识别过程标志物、有风险的个体和新的治疗靶点。
