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追寻反义核酸

In pursuit of antisense.

作者信息

Matteucci M D, Wagner R W

机构信息

Gilead Sciences Inc., Foster City, California 94404, USA.

出版信息

Nature. 1996 Nov 7;384(6604 Suppl):20-2.

PMID:8895596
Abstract

The first generation of antisense oligodeoxynucleotides (ODNs) are now undergoing clinical trials, but their effects may reflect biological activities unrelated to their ability to bind RNA. Nevertheless, preclinical animal studies now suggest that phosphorothioate ODNs may be more permeable in certain animal tissues than in cell culture, raising hopes that antisense mechanisms can be exploited pharmacologically.

摘要

第一代反义寡脱氧核苷酸（ODNs）目前正在进行临床试验，但其效果可能反映的是与其结合RNA能力无关的生物学活性。尽管如此，临床前动物研究现在表明，硫代磷酸酯ODNs在某些动物组织中可能比在细胞培养中更具渗透性，这增加了利用反义机制进行药理学研究的希望。

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1
In pursuit of antisense.追寻反义核酸
Nature. 1996 Nov 7;384(6604 Suppl):20-2.
2
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Radiolabeled oligonucleotides for antisense imaging.用于反义成像的放射性标记寡核苷酸。
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Oxetane modified, conformationally constrained, antisense oligodeoxyribonucleotides function efficiently as gene silencing molecules.氧杂环丁烷修饰的、构象受限的反义寡脱氧核糖核苷酸作为基因沉默分子发挥高效作用。
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Massive and selective delivery of lipid-coated cationic lipoplexes of oligonucleotides targeted in vivo to hepatic endothelial cells.
脂质包被的寡核苷酸阳离子脂质体在体内大量且选择性地递送至肝内皮细胞。
Pharm Res. 2002 May;19(5):676-80. doi: 10.1023/a:1015318415705.
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Design of polyzinc finger peptides with structured linkers.具有结构化连接子的多锌指肽的设计
Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1432-6. doi: 10.1073/pnas.98.4.1432.
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Evidence from immunoneutralization and antisense studies that the inhibitory actions of glucocorticoids on growth hormone release in vitro require annexin 1 (lipocortin 1).免疫中和及反义研究的证据表明，糖皮质激素在体外对生长激素释放的抑制作用需要膜联蛋白1（脂皮质蛋白1）。
Br J Pharmacol. 2000 Dec;131(7):1309-16. doi: 10.1038/sj.bjp.0703694.
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