Shinoda M, Ohe Y, Katakai K, Kabeya K, Watanabe M, Miura T, Ishikawa K
Institute of Experimental Animal Research, Gunma University School of Medicine, Japan.
Neuroendocrinology. 1996 Oct;64(4):268-73. doi: 10.1159/000127127.
To clarify the relationship between neuronal cell degeneration and MHC class-I complex expression, we have immunohistochemically examined the distribution of beta(2)-microglobulin in the hypophysectomized rat hypothalamus. In the sham-operated control rats, positive stainings were distributed only in blood vessels in the hypothalamic areas where magnocellular neurons were localized. Three days after hypophysectomy, positive stainings appeared in a large number of magnocellular neurons in the paraventricular and supraoptic nuclei. Most of such beta(2)-microglobulin-positive cells were simultaneously stained with antivasopressin serum. The pattern of distribution of positive cells and the intensity of the stainings remained unchanged at least until the 14th day. These morphological findings suggest that the process of degeneration of hypothalamic magnocellular neurons after hypophysectomy is a useful model to investigate the role of MHC class I complex in the brain.
为阐明神经元细胞变性与MHC I类复合体表达之间的关系，我们采用免疫组织化学方法检测了垂体切除大鼠下丘脑β2-微球蛋白的分布情况。在假手术对照组大鼠中，阳性染色仅分布于下丘脑大细胞神经元所在区域的血管中。垂体切除术后3天，室旁核和视上核中的大量大细胞神经元出现阳性染色。大多数此类β2-微球蛋白阳性细胞同时被抗加压素血清染色。阳性细胞的分布模式和染色强度至少在第14天前保持不变。这些形态学发现表明，垂体切除术后下丘脑大细胞神经元的变性过程是研究MHC I类复合体在脑中作用的有用模型。
