Cillari E, Milano S, D'Agostino P, Arcoleo F, Stassi G, Galluzzo A, Richiusa P, Giordano C, Quartararo P, Colletti P, Gambino G, Mocciaro C, Spinelli A, Vitale G, Mansueto S
Institute of General Pathology, University of Palermo, Italy.
J Infect Dis. 1996 Nov;174(5):1051-7. doi: 10.1093/infdis/174.5.1051.
Interferon (IFN)-gamma, interleukin (IL)-10, IL-6, and tumor necrosis factor (TNF)-alpha were significantly increased in sera from Sicilian patients with acute boutonneuse fever (BF) compared with those of healthy controls. IFN-gamma levels dropped sharply within the second week after infection. IL-6, IL-10, and TNF-alpha levels gradually declined; in convalescent patients only were they in the normal range. In contrast, peripheral blood mononuclear cells (PBMC) stimulated in vitro with phytohemagglutinin (PHA) produced low levels of IL-10 and IFN-gamma in acute BF that were compatible with the reduction in the levels of CD4+, CD4+/CD45RO+, and CD4+/CD45RA+ cells. In vitro production of TNF-alpha and IL-6 from PBMC stimulated with PHA was not significantly modified during the various phases of the infection compared with control PBMC, which could be due to the persistence of high levels of CD14+ monocytes compensating for the decrease in CD20+ B cells.
与健康对照组相比，西西里岛急性纽扣热（BF）患者血清中的干扰素（IFN）-γ、白细胞介素（IL）-10、IL-6和肿瘤坏死因子（TNF）-α显著升高。感染后第二周内，IFN-γ水平急剧下降。IL-6、IL-10和TNF-α水平逐渐下降；仅在恢复期患者中它们才处于正常范围。相比之下，在急性BF中，用植物血凝素（PHA）体外刺激的外周血单核细胞（PBMC）产生低水平的IL-10和IFN-γ，这与CD4 +、CD4 + / CD45RO +和CD4 + / CD45RA +细胞水平的降低相一致。与对照PBMC相比，在感染的各个阶段，PHA刺激的PBMC体外产生TNF-α和IL-6没有明显改变，这可能是由于高水平的CD14 +单核细胞持续存在，弥补了CD20 + B细胞的减少。
