Capala J, Makar M S, Coderre J A
Medical Department, Brookhaven National Laboratory, Upton, New York 11973, USA.
Radiat Res. 1996 Nov;146(5):554-60.
The short (< 10 microns) ranges of alpha and 7Li particles produced during boron neutron capture therapy (BNCT) make the partitioning of the boronated drug within and without the cell of critical importance. The evaluation of the potential usefulness of a boron-containing substance for BNCT requires information about its intracellular accumulation. In the present report, an in vitro method is described for direct measurement of intracellular boron based on rapid centrifugation of cells through a layer of mineral oil and silicon oil to strip away extracellular growth medium. The intracellular concentrations of boronophenylalanine (BPA), mercaptoborane (BSH) and horic acid in malignant cells and in normal cells have been compared. The accumulation ratio is defined as the ratio of the intracellular to the extracellular boron concentration. Boric acid showed an accumulation ratio of 1 while the ratios for BSH and BPA were dependent on cell type and tended to be greater for BPA than for BSH in malignant but not in normal cells.
硼中子俘获疗法（BNCT）过程中产生的α粒子和7Li粒子射程较短（<10微米），这使得硼化药物在细胞内外的分配至关重要。评估含硼物质对BNCT的潜在效用需要了解其细胞内蓄积情况。在本报告中，描述了一种体外方法，该方法基于通过一层矿物油和硅油对细胞进行快速离心以去除细胞外生长培养基，从而直接测量细胞内硼含量。比较了恶性细胞和正常细胞中硼苯丙氨酸（BPA）、巯基硼烷（BSH）和硼酸的细胞内浓度。蓄积率定义为细胞内硼浓度与细胞外硼浓度之比。硼酸的蓄积率为1，而BSH和BPA的蓄积率取决于细胞类型；在恶性细胞中，BPA的蓄积率往往高于BSH，但在正常细胞中并非如此。
