Shimizu A, Berkhoff H A, Kloos W E, George C G, Ballard D N
Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University, Japan.
Am J Vet Res. 1996 Oct;57(10):1458-62.
To investigate the degree of polymorphism in the pulsed-field gel electrophoresis (PFGE) patterns of Staphylococcus intermedius and to assess the value of this typing method for discriminating strains.
52 S intermedius isolates from diseased and healthy dogs.
Chromosomal DNA of S intermedius was digested with restriction endonuclease Sma I, and the fragments were separated by PFGE in a 1% agarose gel.
Sma I cut the chromosomal DNA into 15 to 23 fragments ranging from about < 1 to 679 kb, and most of the detectable fragments were < 155 kb. Nine fragments, 115, 48, 33, 26, 16, 13, 10, 4, and < 1 kb, were shared by all or almost all (> 71%) of the strains examined. Of the 52 strains, each had a different pattern. S intermedius had a high degree of restriction fragment length polymorphism. The PFGE patterns obtained for S intermedius were stable and reproducible when the strains were tested in the different experiments.
Genomic DNA fingerprinting by PFGE is an effective technique for discriminating S intermedius strains. The PFGE method appears to be a useful molecular marker for epidemiologic or ecologic studies of S intermedius.
研究中间葡萄球菌脉冲场凝胶电泳（PFGE）图谱的多态性程度，并评估该分型方法在区分菌株方面的价值。
从患病和健康犬只中分离出的52株中间葡萄球菌。
用限制性内切酶Sma I消化中间葡萄球菌的染色体DNA，片段在1%琼脂糖凝胶中通过PFGE分离。
Sma I将染色体DNA切割成15至23个片段，大小约为<1至679 kb，大多数可检测片段<155 kb。9个片段，115、48、33、26、16、13、10、4和<1 kb，为所有或几乎所有（>71%）检测菌株所共有。52株菌株中，每株都有不同的图谱。中间葡萄球菌具有高度的限制性片段长度多态性。当在不同实验中对菌株进行检测时，中间葡萄球菌获得的PFGE图谱稳定且可重复。
通过PFGE进行基因组DNA指纹图谱分析是区分中间葡萄球菌菌株的有效技术。PFGE方法似乎是中间葡萄球菌流行病学或生态学研究的有用分子标记。
