Wakita Yoshihisa, Shimizu Akira, Hájek Václav, Kawano Junichi, Yamashita Kenji
Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University, Kobe-shi, Hyogo, Japan.
J Vet Med Sci. 2002 Mar;64(3):237-43. doi: 10.1292/jvms.64.237.
Staphylococcus intermedius from pigeons, dogs, foxes, mink, and horses, was characterized by pulsed-field gel electrophoresis (PFGE) to evaluate the use of this typing method for discriminating among strains. SmaI cut the chromosomal DNA into 7-13 fragments ranging from approximately 48 kb to 655 kb, with most of the detectable fragments being smaller than 172 kb. S. intermedius from various animals had a high degree of restriction fragment length polymorphism. Pigeon strains have a similar genotype, despite the difference in their isolation area. Phage typing indicated that the dog, fox, and mink strains belong to the canine I or canine II type. The PFGE method further differentiated the mink strains from the dog and fox strains with regard to three fragments between 256 kb and 570 kb. As such, genomic DNA fingerprinting by PFGE appears to be an effective technique for discriminating S. intermedius strains from various animals. A combination of PFGE typing and phage typing would provide more detailed information than the single method for ecological investigations of S. intermedius.
利用脉冲场凝胶电泳（PFGE）对来自鸽子、狗、狐狸、水貂和马的中间葡萄球菌进行分型，以评估该分型方法在区分菌株方面的应用。SmaI将染色体DNA切割成7 - 13个片段，大小约为48 kb至655 kb，大多数可检测片段小于172 kb。来自不同动物的中间葡萄球菌具有高度的限制性片段长度多态性。尽管鸽子菌株的分离区域不同，但它们具有相似的基因型。噬菌体分型表明，狗、狐狸和水貂菌株属于犬I型或犬II型。PFGE方法在256 kb至570 kb之间的三个片段上进一步区分了水貂菌株与狗和狐狸菌株。因此，PFGE进行基因组DNA指纹图谱分析似乎是区分来自不同动物的中间葡萄球菌菌株的有效技术。与单一方法相比，PFGE分型和噬菌体分型相结合可为中间葡萄球菌的生态学研究提供更详细的信息。
