Shimizu A, Kawano J, Yamamoto C, Kakutani O, Fujita M
Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University, Japan.
Am J Vet Res. 1997 Dec;58(12):1412-6.
To compare pulsed-field gel electrophoresis (PFGE) patterns of Staphylococcus aureus from chickens in England, Belgium, Bulgaria, Argentina, and Japan, to assess the value of PFGE for discriminating strains, and to compare results obtained by PFGE with those obtained by biotyping and phage typing.
78 S aureus isolates from diseased and healthy chickens.
Chromosomal DNA of S aureus was digested with restriction endonuclease Sma I, and fragments were separated by PFGE in 1% agarose gel.
All 78 strains from 5 countries were classified as poultry ecovar according to a previously established biotyping system. Chromosomal DNA was cut by Sma I into 18 to 23 fragments ranging from about 3 to 685 kb. Seventy-eight strains produced 15 types, arbitrarily designated A to O, and 45 subtypes. Some differences were observed in PFGE patterns among countries. However, 10 fragments (333, 190, 110, 63, 55, 42, 34, 19, 10, and 3 kb) were highly conserved and were shared by almost all (> 78%) of the strains examined. The PFGE patterns were compared with those obtained by phage typing. All 29 strains belonging to avian phage-group II produced type A and 19 subtypes. Of the 15 strains belonging to phage-group I, 11 produced 8 types (B to H, O) and 5 subtypes that were different from those of type A.
Genomic DNA fingerprinting by PFGE is an effective technique for discriminating poultry S aureus strains and appears to be a useful method for subtyping strains of avian phage groups or the poultry-specific ecovar.
比较来自英国、比利时、保加利亚、阿根廷和日本鸡群中金黄色葡萄球菌的脉冲场凝胶电泳（PFGE）图谱，评估PFGE区分菌株的价值，并将PFGE结果与生物分型和噬菌体分型结果进行比较。
从患病和健康鸡中分离出的78株金黄色葡萄球菌。
用限制性内切酶Sma I消化金黄色葡萄球菌的染色体DNA，片段在1%琼脂糖凝胶中通过PFGE分离。
根据先前建立的生物分型系统，来自5个国家的所有78株菌株均被归类为家禽生态型。染色体DNA被Sma I切割成18至23个片段，大小约为3至685 kb。78株菌株产生了15种类型，任意命名为A至O，以及45种亚型。各国之间在PFGE图谱上观察到一些差异。然而，10个片段（333、190、110、63、55、42、34、19、10和3 kb）高度保守，几乎所有（>78%）检测菌株都有这些片段。将PFGE图谱与噬菌体分型结果进行比较。属于禽噬菌体II组的所有29株菌株产生A类型和19种亚型。在属于噬菌体I组的15株菌株中，11株产生了8种类型（B至H、O）和5种与A类型不同的亚型。
通过PFGE进行基因组DNA指纹分析是区分家禽金黄色葡萄球菌菌株的有效技术，似乎也是对禽噬菌体组或家禽特异性生态型菌株进行亚型分型的有用方法。
