Jacobowitz D M, Winsky L, Detera-Wadleigh S D
Laboratory of Clinical Science, NIMH, Bethesda, MD 20892-1266, USA.
Histochem Cell Biol. 1996 Sep;106(3):303-10. doi: 10.1007/BF02473240.
The cellular localization of microtubule-associated protein (MAP) 2 kinase mRNA in rat brain was examined by in situ hybridization histochemistry using a synthetic oligonucleotide probe. MAP 2 kinase was expressed in both neuronal and non-neuronal cells. Areas of high density of mRNA label by the MAP 2 kinase probe appeared to be associated with high cellular packing density. Thus, MAP 2 kinase expression was particularly high in regions such as the locus coeruleus, the piriform cortex, the dentate gyrus granule cell layer, pyramidal cells of the hippocampus, the mitral cells of the olfactory bulb, and the large motor neurons of the V and VII nerves. This apparent ubiquitous distribution suggests an important role of MAP 2 kinase in the cellular functions in most cells of the adult brain.
使用合成寡核苷酸探针，通过原位杂交组织化学方法检测了大鼠脑中微管相关蛋白（MAP）2激酶mRNA的细胞定位。MAP 2激酶在神经元细胞和非神经元细胞中均有表达。MAP 2激酶探针标记的mRNA高密度区域似乎与高细胞堆积密度相关。因此，MAP 2激酶在诸如蓝斑、梨状皮质、齿状回颗粒细胞层、海马锥体细胞、嗅球的二尖瓣细胞以及V和VII神经的大型运动神经元等区域表达特别高。这种明显的广泛分布表明MAP 2激酶在成年大脑大多数细胞的细胞功能中起重要作用。
