Furuyama T, Iwahashi Y, Tano Y, Takagi H, Inagaki S
First Department of Anatomy, Osaka City University Medical School, Japan.
Brain Res Mol Brain Res. 1994 Oct;26(1-2):331-6. doi: 10.1016/0169-328x(94)90107-4.
We studied the localization of 63-kDa Ca2+/calmodulin stimulated phosphodiesterase mRNA in the rat brain by in situ hybridization histochemistry using an oligonucleotides probe specific to this enzyme. The signals were especially concentrated in several brain regions such as the olfactory tubercle, accumbens nucleus, caudate putamen, fundus striati, dentate gyrus of the hippocampus, pontine nuclei and dorsal tegmental nucleus. These results suggest that in the neuronal groups containing the strong signals this enzyme is involved in calcium-dependent signal transduction system coupled to cyclic nucleotides messenger systems.
我们使用针对该酶的寡核苷酸探针，通过原位杂交组织化学方法研究了大鼠脑中63-kDa钙调蛋白刺激的磷酸二酯酶mRNA的定位。信号特别集中在几个脑区，如嗅结节、伏隔核、尾状壳核、纹状体底、海马齿状回、脑桥核和背侧被盖核。这些结果表明，在含有强信号的神经元群体中，该酶参与了与环核苷酸信使系统偶联的钙依赖性信号转导系统。
