水在Hin重组酶与DNA识别中的作用。
Participation of water in Hin recombinase--DNA recognition.
作者信息
Robinson C R, Sligar S G
机构信息
Department of Biochemistry, University of Illinois at Urbana-Champaign 61801, USA.
出版信息
Protein Sci. 1996 Oct;5(10):2119-24. doi: 10.1002/pro.5560051019.
Abstract
The participation of water molecules in the interaction between the Hin recombinase and its operator DNA has been detected by analysis of the dissociation constant in the presence of varying concentrations of neutral solutes and cosolvents. The dissociation constant as measured by gel mobility shift assays increased as the concentration of dimethyl sulfoxide, glycerol, sucrose, or polyethylene glycol was increased. Osmotic pressure is the only property that correlates with the change in the dissociation constant for all compounds. This data indicates that binding of a small population of water molecules accompanies formation of the Hin-DNA complex, and points to a novel role for solvent molecules in assisting site specific interaction between DNA-binding proteins and their cognate DNA sequence.
摘要
通过分析在不同浓度中性溶质和共溶剂存在下的解离常数，检测到水分子参与Hin重组酶与其操纵子DNA之间的相互作用。通过凝胶迁移率变动分析测定的解离常数随着二甲基亚砜、甘油、蔗糖或聚乙二醇浓度的增加而增大。渗透压是与所有化合物解离常数变化相关的唯一性质。该数据表明，一小部分水分子的结合伴随着Hin-DNA复合物的形成，并指出溶剂分子在协助DNA结合蛋白与其同源DNA序列之间的位点特异性相互作用中具有新的作用。
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2
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3
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Biochemistry. 1993 Feb 9;32(5):1354-63. doi: 10.1021/bi00056a022.
4
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5
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6
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7
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