Feldman D A, Weinhold P A
Biochemistry. 1977 Jul 26;16(15):3470-5. doi: 10.1021/bi00634a028.
A calcium binding lipoprotein component of rat heart plasma membrane was isolated, purified, and characterized. The lipoprotein complex had an apparent molecular weight of 71 400. One mole of lipoprotein contained 90 mol of phospholipid and 1 mol of a 12 300 molecular weight protein. The maximum calcium binding capacity was 4.27 mu mol/mg of protein, which corresponded to 52 mol of calcium per mol of lipoprotein complex. Calcium binding was competitively inhibited by a variety of metal ions and experimental antiarrhythmic and anesthetic agents.
大鼠心脏质膜的一种钙结合脂蛋白成分被分离、纯化并进行了表征。该脂蛋白复合物的表观分子量为71400。一摩尔脂蛋白含有90摩尔磷脂和1摩尔分子量为12300的蛋白质。最大钙结合能力为4.27μmol/mg蛋白质，这相当于每摩尔脂蛋白复合物结合52摩尔钙。多种金属离子以及实验性抗心律失常药和麻醉药可竞争性抑制钙结合。
