Kikuchi N, Hiramune T, Takahashi T, Yanagawa R
Department of epizootiology, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
J Vet Med Sci. 1996 Sep;58(9):915-7. doi: 10.1292/jvms.58.915.
Detection of plasmid from leptospires and a comparison of the plasmid profiles between virulent and avirulent strains were performed to investigate whether leptospires contained plasmid(s) associated with virulence. Virulent strains of Leptospira interrogans serovars copenhageni, lai, canicola and pomona, which were virulent for the guinea pig and/or hamster and which showed chemotaxis toward hemoglobin, contained approximately 370 kilobases (kb) plasmid. Avirulent strains of L. interrogans also contained identical plasmid. Similar plasmid profiles in virulent and avirulent strains of L. interrogans were observed. These data showed that no plasmids associated with virulence or chemotaxis were detected. Strains of saprophytic leptospires, L. biflexa and Leptonema illini, did not possess any plasmid.
从钩端螺旋体中检测质粒，并比较有毒和无毒菌株之间的质粒图谱，以研究钩端螺旋体是否含有与毒力相关的质粒。问号状钩端螺旋体血清型哥本哈根、赖、犬和波摩那的有毒菌株，对豚鼠和/或仓鼠有毒性，且对血红蛋白有趋化性，含有约370千碱基（kb）的质粒。问号状钩端螺旋体的无毒菌株也含有相同的质粒。在问号状钩端螺旋体的有毒和无毒菌株中观察到相似的质粒图谱。这些数据表明，未检测到与毒力或趋化性相关的质粒。腐生钩端螺旋体双曲钩端螺旋体和伊利诺伊细螺旋体菌株不具有任何质粒。
