Hashimoti E, Kojimahara N, Noguchi S, Taniai M, Ishiguro N, Hayashi N
Institute of Gastroenterology, Tokyo Women's Medical College, Japan.
J Clin Gastroenterol. 1996 Oct;23(3):199-202. doi: 10.1097/00004836-199610000-00008.
The distinctive histologic findings in acute hepatitis A, B, and C suggest that different immunological mechanisms are involved in the pathogenesis of these diseases. This study was undertaken to define the immune response in each type of acute hepatitis by identification of the intrahepatic lymphocyte subsets. Thirty paraffin-embedded liver biopsy specimens from 10 patients with acute hepatitis A, 10 patients with acute hepatitis B, and 10 patients with acute hepatitis C were evaluated. Immunohistochemical staining was performed by the indirect immunoperoxidase technique using the following monoclonal or polyclonal antibodies: CD45RO, CD20-cy, CD57, and Mac387. Inflammatory infiltrates varied from specimen to specimen. However, CD45RO+ memory T cells were the predominant infiltrating mononuclear cells in all specimens. In the portal areas, CD45RO+ memory T cells were the most prominent in AHC, followed by AHA and AHB, and the difference between AHC and AHB was statistically significant. CD20-cy+ B cells were seen mainly in the portal areas, and were significantly less common in AHB than in AHA and AHC. In addition, the ratio of CD20-cy+ B cells to CD45RO+ memory was significantly lower in AHB than in the other types of acute hepatitis. The necrotic areas in all specimens contained mainly CD45RO+ memory cells in association with a few CD57+ NK and T cells or CD20-cy+ B cells. Our study revealed differences of the intrahepatic lymphocyte subsets among the various types of acute hepatitis, but the meaning of these differences is presently unknown. Therefore, further studies are required to fully elucidate the mechanism of the immune response in acute hepatitis.
急性甲型、乙型和丙型肝炎独特的组织学表现提示，这些疾病的发病机制涉及不同的免疫机制。本研究旨在通过鉴定肝内淋巴细胞亚群来明确每种急性肝炎类型中的免疫反应。对10例急性甲型肝炎患者、10例急性乙型肝炎患者和10例急性丙型肝炎患者的30份石蜡包埋肝活检标本进行了评估。采用间接免疫过氧化物酶技术，使用以下单克隆或多克隆抗体进行免疫组织化学染色：CD45RO、CD20-cy、CD57和Mac387。不同标本的炎症浸润各不相同。然而，CD45RO + 记忆T细胞是所有标本中主要的浸润单核细胞。在门管区，CD45RO + 记忆T细胞在急性丙型肝炎中最为突出，其次是急性甲型肝炎和急性乙型肝炎，急性丙型肝炎和急性乙型肝炎之间的差异具有统计学意义。CD20-cy + B细胞主要见于门管区，在急性乙型肝炎中比在急性甲型肝炎和急性丙型肝炎中明显少见。此外，急性乙型肝炎中CD20-cy + B细胞与CD45RO + 记忆细胞的比例显著低于其他类型的急性肝炎。所有标本的坏死区域主要含有CD45RO + 记忆细胞，并伴有少量CD57 + 自然杀伤细胞和T细胞或CD20-cy + B细胞。我们的研究揭示了不同类型急性肝炎之间肝内淋巴细胞亚群的差异，但这些差异的意义目前尚不清楚。因此，需要进一步研究以充分阐明急性肝炎免疫反应的机制。
