Govindarajan S, Uchida T, Peters R L
Liver. 1983 Feb;3(1):13-8. doi: 10.1111/j.1600-0676.1983.tb00845.x.
The lymphocytic infiltrates were examined in 10 liver biopsy cores taken from nine cases of acute viral hepatitis (AVH), using cryostat sections. The cells were identified and characterized, using the monoclonal antibodies to T lymphocytes (OKT3), T helper cells (OKT4), and T suppressor/cytotoxic cells (OKT8) in an indirect immunofluorescence technique. Of the nine cases of AVH, three were type B, one was acute type A, with underlying chronic hepatitis type B, and the remaining five were non-A, non-B, with no serological markers. One of the patients with acute non-A, non-B hepatitis was biopsied twice with a 2-month interval due to clinical relapse. Both these biopsies were included in the study. The cellular infiltrates, both lobular and portal, were graded from 1 + to 4 + and evaluated by examining the histological sections stained with hematoxylin and eosin. The approximate percentage of each set of cells staining with different antibodies was estimated in each case. The predominant cells were found to be T lymphocytes in all nine cases of AVH; of the two subsets, T suppressor/cytotoxic cells formed the majority in all except one patient who had AVH type A superimposed on chronic B hepatitis.
采用低温恒冷切片机切片，对取自9例急性病毒性肝炎（AVH）患者的10个肝活检组织芯中的淋巴细胞浸润情况进行了检查。采用间接免疫荧光技术，使用针对T淋巴细胞（OKT3）、T辅助细胞（OKT4）和T抑制/细胞毒性细胞（OKT8）的单克隆抗体对细胞进行鉴定和特征分析。在这9例AVH患者中，3例为B型，1例为急性A型，伴有潜在的慢性B型肝炎，其余5例为非甲非乙型，无血清学标志物。1例急性非甲非乙型肝炎患者因临床复发在2个月的间隔内进行了两次活检。这两次活检均纳入研究。对小叶和汇管区的细胞浸润进行1+至4+分级，并通过检查苏木精和伊红染色的组织学切片进行评估。在每个病例中估计用不同抗体染色的每组细胞的大致百分比。发现在所有9例AVH患者中主要细胞均为T淋巴细胞；在两个亚群中，除1例慢性B型肝炎叠加急性A型肝炎的患者外，T抑制/细胞毒性细胞在所有患者中占多数。
