Rubtsov P M, Efremovich N V, Kulaev I S
Biokhimiia. 1977 May;42(5):890-7.
High-molecular polyphosphates have been identified in a crude fraction of chloroplasts of Acetabularia mediterranea. However, after a short-term treatment of the fraction with a hypotonic salt solution and centrifugation in a sucrose density gradient it was found possible to completely separate high-molecular polyphosphates from intact chloroplasts. Consequently the chloroplasts themselves contain no high-molecular polyphosphates. It is assumed that the high-molecular polysphates found in a crude fraction of chloroplasts are constitutents of the "metachromatic" granules which can be revealed in the A. mediterranea cytoplasm by cytochemical methods.
在地中海伞藻叶绿体的粗提物中已鉴定出高分子量的多磷酸盐。然而,在用低渗盐溶液对该提取物进行短期处理并在蔗糖密度梯度中离心后,发现有可能将高分子量的多磷酸盐与完整的叶绿体完全分离。因此,叶绿体本身不含高分子量的多磷酸盐。据推测,在叶绿体粗提物中发现的高分子量多磷酸盐是“异染性”颗粒的组成成分,这些颗粒可以通过细胞化学方法在地中海伞藻的细胞质中显示出来。