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[通过核移植在伞藻叶绿体组分中诱导苹果酸脱氢酶同工酶]

[Malate dehydrogenase isoenzyme induction in an Acetabularia chloroplast fraction with nuclear transplants].

作者信息

Astaurova O B, Afanasova L A, Iazykov A A

出版信息

Ontogenez. 1977;8(4):429-32.

PMID:904860
Abstract

Changes in isozymes of malate dehydrogenase (MDH) in the chloroplast fraction under the transplantation of the A. crenulata nuclei in the A. mediterranea anuclear fragments were studied by means of electrophoresis in polyacrilamide gel. Within 30 days after the transplantation the nucleus induces in the chloroplast fraction MDH isozymes similar by the electrophoretic mobility with those of A. crenulata. The induction of such isozymes was observed both in the plants with the morphological characters of A. crenulata and the fragments which have not formed the reproductive caps, as well as under the transplantation of a small amount of cytoplasm from A. crenulata with chloroplasts in the anuclear fragments of A. mediterranea.

摘要

通过聚丙烯酰胺凝胶电泳法,研究了在地中海无核片段中移植圆齿鹿角菜细胞核后叶绿体部分苹果酸脱氢酶(MDH)同工酶的变化。移植后30天内,细胞核在叶绿体部分诱导出电泳迁移率与圆齿鹿角菜相似的MDH同工酶。在具有圆齿鹿角菜形态特征的植株和未形成生殖帽的片段中均观察到了这种同工酶的诱导现象,以及在地中海无核片段中移植少量带有叶绿体的圆齿鹿角菜细胞质时也观察到了这种现象。

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