Sainio-Pöllänen S, Rulli M, Pöllänen P, Simell O
Department of Pediatrics, University of Turku, Finland.
Pancreas. 1996 Nov;13(4):388-94. doi: 10.1097/00006676-199611000-00008.
The immunopathogenetic mechanisms that lead to type I diabetes in the nonobese diabetic mouse are poorly defined. The immunoregulatory effects of the costimulatory CD80/B7-1 and CD86/B7-2 antigens expressed by the antigen-presenting cells may be crucial for the development of immune responses and their absence may lead to clonal anergy, which is important in the prevention of autoimmune reactivity. To evaluate the role of the CD80 and CD86 antigens in the development of insulin-dependent diabetes mellitus, expression of these antigens in the pancreas of normal and nonobese diabetic mice was studied. The pancreata of normal BALB/c mice and young nonobese diabetic mice contained no CD80- or CD86-positive cells. CD80- and CD86-positive cells appeared in the pancreas of nonobese diabetic mice by 6 weeks of age. Double immunostaining at the age of 12 weeks showed that CD80-positive cells accumulated around the islets of Langerhans but no insulin-containing cells expressed the costimulatory molecules. The morphology of the CD80- and CD86-positive cells was heterogeneous. Many were Mac-1 integrin (CD11b/CD18) positive, suggesting that macrophages expressing the costimulatory antigens are present in the pancreas of all nonobese diabetic mice by 12 weeks of age and that CD80- and CD86-expressing macrophages may be involved in the local regulation of antiislet immune responses.
导致非肥胖型糖尿病小鼠患I型糖尿病的免疫发病机制尚不清楚。抗原呈递细胞表达的共刺激分子CD80/B7-1和CD86/B7-2抗原的免疫调节作用可能对免疫反应的发展至关重要，其缺失可能导致克隆无能，这在预防自身免疫反应中很重要。为了评估CD80和CD86抗原在胰岛素依赖型糖尿病发病中的作用，研究了这些抗原在正常和非肥胖型糖尿病小鼠胰腺中的表达。正常BALB/c小鼠和幼年非肥胖型糖尿病小鼠的胰腺中没有CD80或CD86阳性细胞。到6周龄时，CD80和CD86阳性细胞出现在非肥胖型糖尿病小鼠的胰腺中。12周龄时的双重免疫染色显示，CD80阳性细胞聚集在胰岛周围，但没有含胰岛素的细胞表达共刺激分子。CD80和CD86阳性细胞的形态是异质性的。许多细胞Mac-1整合素（CD11b/CD18）呈阳性，这表明到12周龄时，表达共刺激抗原的巨噬细胞存在于所有非肥胖型糖尿病小鼠的胰腺中，并且表达CD80和CD86的巨噬细胞可能参与了抗胰岛免疫反应的局部调节。
