Sainio-Pöllänen S, Saari T, Simell O, Pöllänen P
Department of Pediatrics, University of Turku, Finland.
J Reprod Immunol. 1996 Oct;31(3):145-63. doi: 10.1016/0165-0378(96)00983-7.
The expression of two accessory molecules on antigen-presenting cells (APC), the CD80/ B7-1 and CD86/B7-2 antigens, was studied in the testis of normal and non-obese diabetic (NOD) mice. In addition, the effect of CD28 stimulation on suppression of lymphocytes by testicular products was investigated. The testes of 4-week old NOD mice or normal BALB/c mice and the testis of 17-21-week old BALB/c mice contained no CD80 or CD86 expressing cells. In contrast, CD80+ and CD86+ cells were present in the testis of 14-22-week old NOD mice. The CD80+ cells and most of the CD86+ cells were CD11b/CD18 negative. There were some CD11b/CD18+ cells that expressed CD86 weakly. The CD80+ and CD86+ cells were often located adjacent to the vessel walls where a leukocyte not expressing CD80 or CD86 had attached to the endothelium. Some CD80+ and CD86+ cells were present among the interstitial cells. The CD80 and CD86 antigens could not be observed in the same cells as judged from stainings in parallel sections. Stimulation of ConA- or anti-CD3 epsilon-primed peripheral blood or spleen lymphocytes with anti-CD28 was able significantly to antagonize the growth-inhibitory effect of the M(r) > 5 K fraction of testis extracts, but could not abolish it with increasing concentrations of testis extract. The results suggest that T lymphocytes can not be activated locally in the testis of BALB/c and young NOD mice because of the absence of the necessary CD28 ligands, CD80 and CD86, from the APCs and because of the suppression of T lymphocytes by the testicular products. In the testis of older diabetic NOD mice lymphocyte activation may occur because the testes of these mice contain CD80+; CD11b/CD18-, CD86+; CD11b/CD18+ and CD86+; CD11b/CD18- cells and therefore, CD28 co-stimulation, which can antagonize the suppressive effect of testis extract, may occur. The possibilities for clonal anergy in testicular immunoregulation are discussed.
在正常和非肥胖型糖尿病（NOD）小鼠的睾丸中，研究了抗原呈递细胞（APC）上两种辅助分子CD80/B7-1和CD86/B7-2抗原的表达情况。此外，还研究了CD28刺激对睾丸产物抑制淋巴细胞作用的影响。4周龄NOD小鼠或正常BALB/c小鼠的睾丸以及17 - 21周龄BALB/c小鼠的睾丸中，未发现表达CD80或CD86的细胞。相比之下，14 - 22周龄NOD小鼠的睾丸中存在CD80+和CD86+细胞。CD80+细胞和大多数CD86+细胞CD11b/CD18呈阴性。有一些CD11b/CD18+细胞弱表达CD86。CD80+和CD86+细胞常位于血管壁附近，此处有一个未表达CD80或CD86的白细胞附着在内皮上。间质细胞中也存在一些CD80+和CD86+细胞。从平行切片染色判断，CD80和CD86抗原不在同一细胞中。用抗CD28刺激经刀豆蛋白A（ConA）或抗CD3ε预刺激的外周血或脾淋巴细胞，能够显著拮抗睾丸提取物分子量>5k组分的生长抑制作用，但随着睾丸提取物浓度增加并不能完全消除这种作用。结果表明，由于APC中缺乏必要的CD28配体CD80和CD86，以及睾丸产物对T淋巴细胞的抑制作用使得BALB/c和幼年NOD小鼠睾丸中的T淋巴细胞无法在局部被激活。在年龄较大的糖尿病NOD小鼠睾丸中可能发生淋巴细胞激活，因为这些小鼠的睾丸含有CD80+；CD11b/CD18-、CD86+；CD11b/CD18+和CD86+；CD11b/CD18-细胞，因此可能发生可拮抗睾丸提取物抑制作用的CD28共刺激。文中还讨论了睾丸免疫调节中克隆无能的可能性。
