Makino Y, Minamino N, Kakishita E, Kangawa K, Matsuo H
National Cardiovascular Center Research Institute, Osaka, Japan.
Peptides. 1996;17(6):1031-9. doi: 10.1016/0196-9781(96)00148-9.
To elucidate the differences of physiological function of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP), we measured tissue concentration and mRNA level in cardiac atrium, ventricle, and brain as well as plasma concentration of these three peptides in water-deprived (WD) and in salt-loaded (SI.) rats. WD rats were given no water for 5 days, whereas SL rats had free access to 2% saline for 14 days. Plasma ANP and BNP concentration of the WD group decreased to 21% and < 10%, and the concentration of the SL group to 36% and 47% of the control (CON) group. Atrial and ventricular BNP concentration of the WD group decreased to 36% and 23% of the CON group, and atrial BNP concentration of the SL group decreased to a lesser extent. BNP mRNA level in atrium and ventricle decreased in both the WD and SL groups, with the WD group showing larger decreases to 11% and 20% of the CON group. ANP mRNA level in atrium and ventricle of the WD group decreased to 40% and 61% of the CON group, but increased to 147% in ventricle of the SL group. In the brain, no significant change was observed in ANP and CNP concentration in both the WD and SL group. Under these conditions. BNP generally showed larger changes than ANP in both peptide concentration and mRNA level. Gene transcription and peptide production of ANP and BNP in atrium and in ventricle were not always found to be in concert, especially in the case of salt loading. Taken as a whole, our results demonstrate differences among the systems regulating biosynthesis and secretion of ANP and BNP in atrium and ventricle.
为阐明心房利钠肽（ANP）、脑利钠肽（BNP）和C型利钠肽（CNP）生理功能的差异，我们测定了禁水（WD）和高盐负荷（SL）大鼠心脏心房、心室及脑内这三种肽的组织浓度和mRNA水平，以及血浆浓度。WD大鼠禁水5天，而SL大鼠自由饮用2%盐水14天。WD组血浆ANP和BNP浓度分别降至对照组（CON）的21%和<10%，SL组则降至36%和47%。WD组心房和心室BNP浓度分别降至CON组的36%和23%，SL组心房BNP浓度下降幅度较小。WD组和SL组心房及心室内BNP mRNA水平均下降，WD组下降幅度更大，分别降至CON组的11%和20%。WD组心房和心室内ANP mRNA水平分别降至CON组的40%和61%，但SL组心室内ANP mRNA水平升至147%。在脑中，WD组和SL组ANP和CNP浓度均未观察到显著变化。在这些条件下，BNP在肽浓度和mRNA水平方面的变化通常比ANP更大。心房和心室内ANP和BNP的基因转录和肽产生并不总是一致，尤其是在高盐负荷情况下。总体而言，我们的结果表明心房和心室内调节ANP和BNP生物合成及分泌的系统存在差异。
