Malek A, Miller R K, Mattison D R, Kennedy S, Panigel M, di Sant'Agnese P A, Jessee L
Department of Obstetrics and Gynecology, University of Rochester Medical Center, New York 14642-8668, USA.
Placenta. 1996 Sep;17(7):495-506. doi: 10.1016/s0143-4004(96)90032-6.
Phosphorus 31 nuclear magnetic resonance spectroscopy as a non-invasive technique was applied to monitor the metabolic activity of the human placenta during perfusion in vitro. During control perfusions (n = 3) there was an initial increase in adenosine triphosphate (ATP) and a fall in inorganic phosphate (Pi). Thereafter, however, the level of both ATP and Pi remained constant throughout the perfusion period (11 h). Additional biochemical parameters such as glucose consumption, lactate production and the release of hormones, human chorionic gonadotrophin (hGC). measured in the perfusate samples, were also used to assess the viability of the placental tissue. As with ATP, all these biochemical parameters under the control conditions showed a stable rate of metabolic activity throughout the length of the experiments. In additional experiments, the effect of the metabolic inhibitor dinitrophenol (n = 2) and dinitrophenol (DNP) together with iodoacetic acid (IOA, n = 2) were studied. DNP (0.1 mM) alone showed a slight decrease of all parameters. In contrast, the addition of IOA (0.1 mM) with DNP (0.1 mM) not only blocked the production of ATP but also produced a substantial impact on placental metabolic activity. The effect of a toxic dose of cadmium (20 nmol/ml) was studied also (n = 3). This dose of cadmium demonstrated no effect on phosphorus metabolism. However, the rate of glucose consumption and the release of hCG were significantly reduced.
磷31核磁共振波谱作为一种非侵入性技术，被应用于体外灌注过程中监测人胎盘的代谢活性。在对照灌注实验（n = 3）中，三磷酸腺苷（ATP）最初增加，无机磷酸盐（Pi）减少。然而，此后在整个灌注期（11小时），ATP和Pi的水平都保持恒定。灌注液样本中测量的其他生化参数，如葡萄糖消耗、乳酸生成和激素人绒毛膜促性腺激素（hCG）的释放，也被用于评估胎盘组织的活力。与ATP一样，在对照条件下，所有这些生化参数在整个实验过程中都显示出稳定的代谢活性速率。在额外的实验中，研究了代谢抑制剂二硝基苯酚（n = 2）以及二硝基苯酚（DNP）与碘乙酸（IOA，n = 2）共同作用的效果。单独使用DNP（0.1 mM）时，所有参数略有下降。相比之下，添加IOA（0.1 mM）与DNP（0.1 mM）不仅阻断了ATP的生成，还对胎盘代谢活性产生了重大影响。还研究了有毒剂量的镉（20 nmol/ml）的作用（n = 3）。该剂量的镉对磷代谢没有影响。然而，葡萄糖消耗率和hCG的释放显著降低。
