Sahenk Z, Mendell J R
Brain Res. 1979 Jul 27;171(1):41-53. doi: 10.1016/0006-8993(79)90730-3.
Fast axoplasmic transport was examined in experimental nerve ligation and adriamycin-induced neuronal perikaryon lesions. Animals were sacrificed at precisely 3 and 4 h following the injection of [3H]leucine. In single ligated nerves, the 3 and 4 h down-flow rates, which include the time for neuronal processing of labelled material, were 379.2 +/- 23.51 and 370.8 +/- 26.93 mm/day, respectively, compared to the calculated rate of fast axoplasmic transport of 345.6 +/- 25.22 mm/day. In double-ligated nerves the down-flow rates were 364.8 +/- 40.32 and 342 +/- 32.2 mm/day, respectively, while the fast axoplasmic transport rate was 273.6 +/- 36.16 mm/day. In contrast, in adriamycin toxicity the down-flow rates were 363.9 +/- 21.54 and 378 +/- 14.69 mm/day with a normal calculated rate of fast axoplasmic transport of 420 +/- 18 mm/day. These studies illustrate that evaluation of two (or more) different time intervals can be used to assess the site of impaired delivery of labelled materials to the peripheral portions of the nerve. Furthermore, a single time interval examination of transport should not be equated with axoplasmic transport especially under pathological conditions.
在实验性神经结扎和阿霉素诱导的神经元胞体损伤中检测了快速轴浆运输。在注射[3H]亮氨酸后精确的3小时和4小时处死动物。在单结扎神经中，包括标记物质在神经元内处理时间的3小时和4小时的下行运输速率分别为379.2±23.51和370.8±26.93毫米/天，而计算出的快速轴浆运输速率为345.6±25.22毫米/天。在双结扎神经中，下行运输速率分别为364.8±40.32和342±32.2毫米/天，而快速轴浆运输速率为273.6±36.16毫米/天。相比之下，在阿霉素毒性中，下行运输速率为363.9±21.54和378±14.69毫米/天，计算出的快速轴浆运输正常速率为420±18毫米/天。这些研究表明，评估两个（或更多）不同的时间间隔可用于评估标记物质向神经外周部分传递受损的部位。此外，特别是在病理条件下，不应将单次运输时间间隔检查等同于轴浆运输。
