洋葱伯克霍尔德菌2a菌株中新型插入序列IS1471的鉴定与测序
Identification and sequencing of a novel insertion sequence, IS1471, in Burkholderia cepacia strain 2a.
作者信息
Xia X S, Smith A R, Bruce I J
机构信息
School of Chemical and Life Sciences, University of Greenwich, London, UK.
出版信息
FEMS Microbiol Lett. 1996 Nov 1;144(2-3):203-6. doi: 10.1111/j.1574-6968.1996.tb08531.x.
A novel insertion sequence (IS), IS1471, has been identified which is inserted into IS element IS1071 possessed by plasmid pIJB1 in Burkholderia cepacia strain 2a. Nucleotide sequencing has revealed that IS1471 is 1112 bp in length and is flanked by 22/21-bp imperfect inverted repeats with a 3-bp duplication of the target sequence IS1471 contains a single open reading frame encoding a putative polypeptide of 345 amino acids with molecular mass of 39406 Da. Searches of DNA and protein sequence databases did not result in the detection of any homologous IS elements, suggesting that IS1471 is novel and may not belong to any known IS groups.
已鉴定出一种新的插入序列（IS），即IS1471，它插入到洋葱伯克霍尔德菌2a菌株中质粒pIJB1所携带的IS元件IS1071中。核苷酸测序表明，IS1471长度为1112 bp，两侧为22/21 bp的不完全反向重复序列，靶序列有3 bp的重复。IS1471包含一个单一的开放阅读框，编码一个推定的由345个氨基酸组成的多肽，分子量为39406 Da。对DNA和蛋白质序列数据库的搜索未检测到任何同源的IS元件，这表明IS1471是新的，可能不属于任何已知的IS组。
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