Lord B I, Testa N G, Wright E G, Banerjee R K
Biomedicine. 1977 May;26(3):163-9.
Using the agar culture technique, we have measured the effect of granulocyte extracts GCE (and of erythrocyte-RCE and lymph node extracts-LNE) on the growth and proliferation of the committed granulocytic precursor cells, CFU-C. In addition we have determined their effects on the proliferation of the developing colony cells and on the ultimate cell production in the colonies. The results show that GCE has no effect on the growth or proliferative activity on the CFU-C. It does, however, reduce both the autoradiographic labelling indices of the developing colony cells and the net colony cellularities, acting as a cell cycle modulator. These are effects specific to the GCE since at the dose levels used, neither RCE nor LNE affected these measurements.
采用琼脂培养技术，我们测定了粒细胞提取物（GCE）以及红细胞提取物（RCE）和淋巴结提取物（LNE）对定向粒细胞前体细胞即集落形成单位（CFU-C）生长和增殖的影响。此外，我们还确定了它们对发育中集落细胞增殖以及集落最终细胞生成的影响。结果表明，GCE对CFU-C的生长或增殖活性没有影响。然而，它确实降低了发育中集落细胞的放射自显影标记指数以及集落的净细胞数，起到细胞周期调节剂的作用。这些是GCE特有的效应，因为在所使用的剂量水平下，RCE和LNE均未影响这些测量结果。
