HOELZER D, HARRISS E B, SLADE M, KURRLE E
J Cell Physiol. 1976 Sep;89(1):89-99. doi: 10.1002/jcp.1040890109.
The growth of granulopoietic progenitor cells (CFU-C) in diffusion chambers during culture of peripheral blood leukocytes from 10 normal subjects has been studied. At various times after initiation of diffusion chamber culture, cells harvested from the chambers were transferred to agar culture for measurement of CFU-C concentration. Under these conditions colonies could be grown successfully in agar culture provided pronase, necessary for the chamber harvesting procedure, was first removed by careful washing. A marked increase in the number of CFU-C, up to 25-fold the initial value, was observed in 8 out of 10 subjects. Here the growth pattern was similar, independent of the initial CFU-C throughout the diffusion chamber culture period was very poor. The growth of CFU-C from a given individual's blood was shown to be reproducible in repeated studies in 2 subjects, one of whom showed a proliferative and the other a non-proliferative pattern. Evidence suggests that the increase in CFU-C in diffusion chambers is the result of both self-renewal of these cells and influx from a more primitive compartment, although the present data do not allow an estimate of the relative magnitude of each.
对10名正常受试者外周血白细胞进行扩散盒培养时，研究了粒系祖细胞（CFU-C）的生长情况。在扩散盒培养开始后的不同时间，从盒中收获的细胞被转移至琼脂培养中，以测定CFU-C浓度。在这些条件下，只要在收获过程中必需的链霉蛋白酶通过仔细洗涤预先去除，就能在琼脂培养中成功培养出集落。10名受试者中有8名观察到CFU-C数量显著增加，增至初始值的25倍。这里的生长模式相似，在整个扩散盒培养期间，与初始CFU-C无关的生长情况非常差。在对2名受试者的重复研究中表明，来自给定个体血液的CFU-C生长具有可重复性，其中一名受试者呈现增殖模式，另一名呈现非增殖模式。有证据表明，扩散盒中CFU-C的增加是这些细胞自我更新以及来自更原始细胞区室流入的结果，尽管目前的数据无法估计各自的相对程度。
