Faille A, Dresch C, Poirier O, Najean Y
Biomedicine. 1977 May;26(3):187-94.
Granulopoiesis was studied in 20 cases of chronic granulocytopenia (adults and children; acquired and congenital). Bone marrow colony growth in methylcellulose culture was compared with the turnover-rate of blood granulocytes labelled with 51Cr and with the bone marrow granulocyte labelling index (L.I.) after "in vitro" flash labelling with 3H-thymidine (3H-TDR). There was a positive correlation between colony forming cells (C.F.C.) content and turnover rate and between C.F.C. content and bone marrow cellularity but there was no statistically valid correlation between C.F.C. content and labelling index. In patients with acquired granulocytopenia the colony counts were increased in cases with autoimmune destruction of neutrophils and in one patient during granulocytic regeneration. The C.F.C. numbers were low in cases with neutropenia associated with granulocyte hypoplasia but the prognosis was better in those cases with the highest colony count. On the other hand, colony counts were variable and had no prognostic value in cases of neutropenia secondary to a bone marrow abnormality in maturation or mutiplication, either acquired or congenital, as revealed by turnover studies and autoradiographic data.
对20例慢性粒细胞减少症患者（成人和儿童；后天性和先天性）的粒细胞生成进行了研究。将甲基纤维素培养中的骨髓集落生长与用51Cr标记的血液粒细胞周转率以及用3H-胸腺嘧啶核苷（3H-TDR）“体外”快速标记后的骨髓粒细胞标记指数（L.I.）进行了比较。集落形成细胞（C.F.C.）含量与周转率之间以及C.F.C.含量与骨髓细胞密度之间存在正相关，但C.F.C.含量与标记指数之间无统计学上有效的相关性。在后天性粒细胞减少症患者中，中性粒细胞自身免疫性破坏的病例以及1例粒细胞再生期间的患者，集落计数增加。粒细胞发育不全相关中性粒细胞减少症患者的C.F.C.数量较低，但集落计数最高的那些病例预后较好。另一方面，正如周转率研究和放射自显影数据所显示的，在后天性或先天性成熟或增殖骨髓异常继发的中性粒细胞减少症病例中，集落计数变化不定且无预后价值。
