视黄酸和丙戊酸盐对海胆发育的影响。
Effect of retinoic acid and valproate on sea urchin development.
作者信息
Sconzo G, Fasulo G, Romancino D, Cascino D, Giudice G
机构信息
Dipartimento di Biologia Cellulare e dello Sviluppo Alberto Monroy, Università die Palermo, Italy.
出版信息
Pharmazie. 1996 Mar;51(3):175-80.
The effect of valproate and retinoic acid on Paracentrotus lividus development and fertilization is reported. Retinoic acid at concentrations between 5 microM and 7 microM causes severe disturbance of development and at 10 microM complete embryo degeneration if added in the period from fertilization on. Concentrations up to 7 microM are uneffective if retinoic acid addition is started 16 h after fertilization. Valproate at concentrations between 1 mM and 5 mM causes severe disturbances of development if added from 5 min after fertilization on. Concentrations of 20 mM are lethal to all embryos. If the drug is added 16 h after fertilization no effect is observed up to the concentration of 10 mM. The effect of valproate is specific, because its isomere 2-en at 10 mM has very little effect on development. Both drugs affect fertilization only at higher concentrations: more than 1 microM for retinoic acid and more than 10 mM for valproate.
本文报道了丙戊酸盐和视黄酸对紫球海胆发育及受精的影响。若在受精后立即添加视黄酸，浓度在5微摩尔至7微摩尔之间会导致发育严重紊乱，浓度为10微摩尔时会使胚胎完全退化。若在受精16小时后开始添加视黄酸，浓度高达7微摩尔也无影响。若在受精后5分钟开始添加丙戊酸盐，浓度在1毫摩尔至5毫摩尔之间会导致发育严重紊乱。浓度为20毫摩尔时对所有胚胎均有致死作用。若在受精16小时后添加该药物，浓度达10毫摩尔时也无影响。丙戊酸盐的作用具有特异性，因为其异构体2 -烯在浓度为10毫摩尔时对发育几乎没有影响。两种药物均仅在较高浓度下影响受精：视黄酸浓度超过1微摩尔，丙戊酸盐浓度超过10毫摩尔。
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